Development and evaluation of genome-probing microarrays for monitoring lactic acid bacteria = 김치 내 유산균 발효과정 모니터링을 위한 Genome-Probing Microarrays(GPM)의 개발

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dc.contributor.authorJin-Woo Bae-
dc.contributor.authorS K Rhee-
dc.contributor.authorJa Ryeong Park-
dc.contributor.authorWon Hyong Chung-
dc.contributor.authorYoung Do Nam-
dc.contributor.authorI Lee-
dc.contributor.authorH Kim-
dc.contributor.authorYong Ha Park-
dc.date.accessioned2017-04-19T09:03:45Z-
dc.date.available2017-04-19T09:03:45Z-
dc.date.issued2005-
dc.identifier.issn0099-2240-
dc.identifier.uri10.1128/AEM.71.12.8825-8835.2005ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7214-
dc.description.abstractThe genome-probing microarray (GPM) was developed for quantitative, high-throughput monitoring of community dynamics in lactic acid bacteria (LAB) fermentation through the deposit of 149 microbial genomes as probes on a glass slide. Compared to oligonucleotide microarrays, the specificity of GPM was remarkably increased to a species-specific level. GPM possesses about 10- to 100-fold higher sensitivity (2.5 ng of genomic DNA) than the currently used 50-mer oligonucleotide microarrays. Since signal variation between the different genomes was very low compared to that of cDNA or oligonucleotide-based microarrays, the capacity of global quantification of microbial genomes could also be observed in GPM hybridization. In order to assess the applicability of GPMs, LAB community dynamics were monitored during the fermentation of kimchi, a traditional Korean food. In this work, approximately 100 diverse LAB species could be quantitatively analyzed as actively involved in kimchi fermentation.-
dc.publisherAmer Soc Microb-
dc.titleDevelopment and evaluation of genome-probing microarrays for monitoring lactic acid bacteria = 김치 내 유산균 발효과정 모니터링을 위한 Genome-Probing Microarrays(GPM)의 개발-
dc.title.alternativeDevelopment and evaluation of genome-probing microarrays for monitoring lactic acid bacteria-
dc.typeArticle-
dc.citation.titleApplied and Environmental Microbiology-
dc.citation.number12-
dc.citation.endPage8835-
dc.citation.startPage8825-
dc.citation.volume71-
dc.contributor.affiliatedAuthorJin-Woo Bae-
dc.contributor.affiliatedAuthorJa Ryeong Park-
dc.contributor.affiliatedAuthorWon Hyong Chung-
dc.contributor.affiliatedAuthorYoung Do Nam-
dc.contributor.affiliatedAuthorYong Ha Park-
dc.contributor.alternativeName배진우-
dc.contributor.alternativeName이성근-
dc.contributor.alternativeName박자령-
dc.contributor.alternativeName정원형-
dc.contributor.alternativeName남영도-
dc.contributor.alternativeName이인순-
dc.contributor.alternativeName김홍익-
dc.contributor.alternativeName박용하-
dc.identifier.bibliographicCitationApplied and Environmental Microbiology, vol. 71, no. 12, pp. 8825-8835-
dc.identifier.doi10.1128/AEM.71.12.8825-8835.2005-
dc.description.journalClassY-
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