Molecular identification of toxic Alexandrium tamiyavanichii (Dinophyceae) using two DNA probes

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Title
Molecular identification of toxic Alexandrium tamiyavanichii (Dinophyceae) using two DNA probes
Author(s)
Choong-Jae Kim; Y Sako
Bibliographic Citation
Harmful Algae, vol. 4, no. 6, pp. 984-991
Publication Year
2005
Abstract
To improve labeling-intensity of whole-cell fluorescence in situ hybridization (FISH) in the molecular identification of toxic Alexandrium tamiyavanichii, two DNA probes (TAMID2 plus TAMIS1 designed from the LSU and SSU rDNA regions, respectively) were used to test the labeling intensity of targeted cultured A. tamiyavanichii cells. The cross-reactivity of the DNA probe to natural seawater samples and six Alexandrium species: A. affine, A. catenella, A. fraterculus, A. insuetum, A. pseudogonyaulax and A. tamarense, was also tested. The labeling intensity of the DNA probe TAMID2S1, a combination of two separate probes that target different regions of the rRNA, was 1.7-2.7 times higher than that of the single DNA probe TAMID2. With cultured A. tamiyavanichii cells in the dead growth phase at 30 days, the TAMID2S1 intensity was 1.9 times higher than that of TAMID2. During a 30-day culture, the labeling intensity of A. tamiyavanichii cells hybridized with TAMID2S1 decreased to 49.4% of the original intensity. No cross-reactivity to various microorganisms in natural seawater samples was found. The two DNA probes together, designated as TAMID2S1, readily detected A. tamiyavanichii added to natural seawater samples, even aged cultured cells.
Keyword
Paralytic shellfish poisoning (PSP)DNA probeFluorescence in situ hybridization (FISH)Alexandrium tamiyavanichii
ISSN
1568-9883
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.hal.2005.01.003
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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