Transcriptome analysis of human gastric cancer

Cited 23 time in scopus
Metadata Downloads
Title
Transcriptome analysis of human gastric cancer
Author(s)
Jung Hwa Oh; Jin Ok Yang; Y Hahn; Mirang Kim; Sang-Soon Byun; Jeo-Jin Jeon; Jeong-Min Kim; K S Song; S M Noh; S Kim; Hyang Sook Yoo; Yong Sung Kim; Nam-Soon Kim
Bibliographic Citation
Mammalian Genome, vol. 16, pp. 942-945
Publication Year
2005
Abstract
To elucidate the genetic events associated with gastric cancer, 124,704 cDNA clones were collected from 37 human gastric cDNA libraries, including 20 full-length enriched cDNA libraries of gastric cancer cell lines and tissues from Korean patients. An analysis of the collected ESTs revealed that 97,930 high-quality ESTs coalesced into 13,001 clusters, of which 11,135 clusters (85.6%) were annotated to known ESTs. The analysis of the full-length cDNAs also revealed that 4862 clusters (51.7%) contained at least one putative full-length cDNA clone with an initiation codon, with the average length of the 5′ UTR of 140 bp. A large number appear to have a diverse transcription start site (TSS). An examination of the TSS of some genes, such as TEGT and GAPD, using 5′ RACE revealed that the predicted TSSs are actually found in human gastric cancer cells and that several TSSs differ depending on the specific gastric cell line. Furthermore, of the human gastric ESTs, 766 genes (9.5%) were present as putative alternatively spliced variants. Confirmation of the predicted spliced isoforms using RT-PCR showed that the predicted isoforms exist in gastric cancer cells and some isoforms coexist in gastric cell lines. These results provide potentially useful information for elucidating the molecular mechanisms associated with gastric oncogenesis.
ISSN
0938-8990
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00335-005-0075-2
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.