Dehydrotrametenolic acid selectively inhibits the growth of H-ras transformed rat2 cells and induces apoptosis through caspase-3 pathway = Dehydrotrametenolic acid의 세포사멸 유도를 통한 항암효과

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Title
Dehydrotrametenolic acid selectively inhibits the growth of H-ras transformed rat2 cells and induces apoptosis through caspase-3 pathway = Dehydrotrametenolic acid의 세포사멸 유도를 통한 항암효과
Author(s)
Hyun-Mi Kang; Su-Kyung Lee; Dae Seop Shin; Mi-Young Lee; Dong Cho Han; N I Baek; Kwang Hee Son; Byoung-Mog Kwon
Bibliographic Citation
Life Sciences, vol. 78, no. 6, pp. 607-613
Publication Year
2006
Abstract
The screening of natural products that preferentially inhibit growth of H-ras transformed rat2 cells vs. rat2 cells was performed to identify H-ras specific growth inhibitor. A lanostane-type triterpene acid, dehydrotrametenolic acid (3β-hydroxylanosta-7,9(11),24-trien-21-oic acid), was isolated from the sclerotium of Poria cocos (Polyporaceae). Dehydrotrametenolic acid selectively inhibited the growth of H-ras transformed cells with a GI 50 value of 40 μM. FACS analysis indicated that the compound exerted its anti-proliferation effects through cell cycle arrest at G2/M phase and accumulation of sub-G1 population. Dehydrotrametenolic acid-induced apoptosis was further confirmed with chromosomal DNA fragmentation, caspase-3 activation, and degradation of PARP and Lamin A/C degradation. The compound also regulated the expression of H-ras, Akt and Erk, which are the downstream proteins of H-ras signaling pathways. The results suggest that dehydrotrametenolic acid can be a potential anticancer agent against H-ras transformed tumor.
Keyword
ApoptosisCaspase-3Cell cycle arrestDehydrotrametenolic acidH-rasPoria cocos Wolf
ISSN
0024-3205
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.lfs.2005.05.066
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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