Isolation of uncultivated anaerobic thermophiles from compost by supplementing cell extract of Geobacillus toebii in enrichment culture medium =농화배양중 G. toebii세포추출액 첨가로 퇴비에 고온 미생물

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Title
Isolation of uncultivated anaerobic thermophiles from compost by supplementing cell extract of Geobacillus toebii in enrichment culture medium =농화배양중 G. toebii세포추출액 첨가로 퇴비에 고온 미생물
Author(s)
Jin-Woo Bae; S K Rhee; Ja Ryeong Park; Byung Chun Kim; Yong Ha Park
Bibliographic Citation
Extremophiles, vol. 9, no. 6, pp. 477-485
Publication Year
2005
Abstract
Several researchers have reported that microorganisms can be cultivated only in the presence of other microorganisms. We suggest that a portion of uncultivated microorganisms might be cultivated in the presence of cellular components released from bacteria in their natural environments. In this study, the cell extract of Geobacillus toebii was used to enrich uncultivated thermophiles from compost. In the process of enrichment cultures, cell extract supplementation apparently changed the community composition. This change was monitored by PCR-DGGE targeting 16S rRNA gene. Five novel groups of microorganisms (similarity of 16S rRNA gene to the closest relative <96%) were specifically isolated from enrichment cultures by using cell extract-supplemented culture media. Their growth was found to be dependent on the addition of extract of G. toebii. Putting these findings together, we suggest that the extracts of bacteria could be one of the growth factors in the thermal ecosystem with a possibility of extending other ecological niches.
Keyword
Cell extractDGGEGeobacillus toebiiGrowth factorsThermophilesUncultivated microorganisms
ISSN
1431-0651
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00792-005-0467-y
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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