Analysis of microbial community during the anaerobic dechlorination of perchloroethylene and trichloroethylene = Perchloroethylene과 trichloroethylene의 혐기적 탈염소화 및 미생물 군집 분석

Abstract

In this study, the anaerobic enrichment cultivation was performed with the sediments and the dredged soils from the cities of Ulsan, Masan, Yeosu, Gwangyang, Ansan and Seongnam. Acetate as an electron donor and PCE (perchloroethylene) or TCE (trichloroethylene) as an electron acceptor were injected into the serum bottle with an anaerobic medium. After the incubation of 12 weeks, the removal efficiency of PCE was highest at 70% in the treatment with the sediment of Ulsan. Also, the bacterial community structure was analyzed by D-DGGE (double denatured gradient gel electrophoresis) through PCR-based 16S rDNA approaches. The dominant species in the anaerobic enrichment were found to belong to the genus of Desulfovibrio.