Cyanobacterial diversity analysis using cpcBA-Intergenic spacer region = cpcBA-intergenic spacer region을 이용한 cyanobacteria의 다양성 분석

Abstract

The cyanobacterial diversity was analyzed by restriction fragment length polymorphism (RFLP) of PCR-amplified cpcBA-Intergenic Spacer (IGS) genes and cpcBA-IGS gene sequencing with a sample collected at Chuso-ri in Daechung Reservoir on March 15, 2005. The Shannon-Weiner diversity index was 0.65, indicating that the cyanobacterial community structure was simple. PCR-RFLP profiles obtained were Phormidium spp. (58 clones), Anabaena spp. (14 clones), Microcystis spp. (4 clones), Spirulina sp. (1 clone) and uncultured cyanobacteria (2 clones). The PCR-RFLP of cpcBA-IGS revealed that Phormidium spp. and Anabaena spp. dominated in the invested sample. As a consequence, it seems that the analysis of functional genes such as cpcBAIGS can be used for the species identification and community analysis of cyanobacteria.