Production of β-sitosterol by cell suspension culture of Chrysanthemum coronarium L. = 쑥갓세포의 현탁배양에 의한 β-sitosterol 생산

Abstract

β-sitosterol is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for β-sitosterol production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal β-sitosterol production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l KNO3, 1650 mg/l NH4NO3), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of β-sitosterol was 150 μg/g DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum β-sitosterol content of 143.8 μg/g DCW was obtained at an air-flow rate of 100 cc/min.