Combinational effect of moist heating and gamma irradiation on the inactivation of trypsin inhibitory activity in soybean

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Title
Combinational effect of moist heating and gamma irradiation on the inactivation of trypsin inhibitory activity in soybean
Author(s)
P Felipe; Y H Yang; J H Lee; D E Sok; Hyoung-Chin KimWoon Kee Yoon; Hwan Mook Kim; M R Kim
Bibliographic Citation
Food Science and Biotechnology, vol. 14, no. 6, pp. 732-737
Publication Year
2005
Abstract
The combinational effect of gamma irradiation and moist heating on the trypsin inhibitor activity (TIA) in soaked and dried soybeans was evaluated by measuring the inhibition using N-benzoyl-DL-arginine-p-nitroanilide (BAPNA) as substrate. Gamma irradiation significantly decreased the TIA level in soybean at doses above 5 kGy, and the ID50 (the gamma irradiation dose required to reach 50% inhibition) value for TIA was 13.53 kGy. Soaking. prior to gamma irradiation significantly lowered the ID50 to 8.44 kGy, and the soaking process enhanced the efficiency to inactivate TIA by as much as 48%. When soaking prior to gamma irradiation was followed by subsequent mild heating (60 degrees C) process, the IT50 (heating time required to reach the 50% inhibition of TIA) value at even 1 kGy (5.28 min) was greatly reduced by over 50% compared to the level for the no-soaking process. In addition, the activation energy of soaking prior to gamma irradiation at 1 kGy was 2.45 kcal/mole, which was also about 50% lower than the 5.10 kcal/mole of dried soybean gamma-irradiated. Based on these results, soaking prior to gamma irradiation is an effective method for TIA inhibition. Furthermore, a combination of two or more processing methods such as soaking, heating and gamma irradiation is much more effective than any single processing method.
Keyword
soybeantrypsin inhibitory activity (TIA)gamma irradiationsoaking
ISSN
1226-7708
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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