DC Field | Value | Language |
---|---|---|
dc.contributor.author | Y J Seo | - |
dc.contributor.author | M H Jeon | - |
dc.contributor.author | J H Lee | - |
dc.contributor.author | Y J Lee | - |
dc.contributor.author | H J Youn | - |
dc.contributor.author | Jeong Heon Ko | - |
dc.contributor.author | J H Lee | - |
dc.date.accessioned | 2017-04-19T09:03:57Z | - |
dc.date.available | 2017-04-19T09:03:57Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | I000-0028 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7246 | - |
dc.description.abstract | Bis (Bag-3, CAIR), a Bcl-2-interacting protein, promotes the anti-apoptotic activity of Bcl-2 and increased levels of Bis have been observed in several disease models. The involvement of Bcl-2 and some Bcl-2-binding proteins in differentiation has recently been reported. However, the relevance of Bis to cellular differentiation remains unknown. The findings herein show that Bis expression is up-regulated during the differentiation of HL-60 cells. To investigate the effect of Bis expression on differentiation, we established Bis-overexpressing HL-60 cells (HL-60-bis). HL-60-bis cells have a low nuclear: cytoplasmic ratio and indented nucleus in Wright-Giemsa staining, and an increased expression of CD11b in immunofluorescence study, indicating the promotion of differentiation. The overexpression of Bis also resulted in a retarded cell growth rate, accompanied by the accumulation of HL-60 cells at the G0/G1 phase of the cell cycle, which was sustained during the differentiation process. Western blot analysis revealed that the expression of p27, a representative inducer of cell cycle arrest at the G1 phase, was increased 2.5-fold in HL-60-bis cells compared to HL-60-neo cells. These results suggest that the Bis induced growth inhibition of HL-60 cells promotes G0/G1 phase arrest via up-regulation of p27, which seems to be a prerequisite for differentiation. Further studies will be required to define the exact roles of Bis on cellular differentiation more precisely. | - |
dc.publisher | Springer-Nature Pub Group | - |
dc.title | Bis induces growth inhibition and differentiation of HL-60 cells via up-regulation of p27 | - |
dc.title.alternative | Bis induces growth inhibition and differentiation of HL-60 cells via up-regulation of p27 | - |
dc.type | Article | - |
dc.citation.title | Experimental and Molecular Medicine | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 630 | - |
dc.citation.startPage | 624 | - |
dc.citation.volume | 37 | - |
dc.contributor.affiliatedAuthor | Jeong Heon Ko | - |
dc.contributor.alternativeName | 서윤지 | - |
dc.contributor.alternativeName | 전미희 | - |
dc.contributor.alternativeName | 이정희 | - |
dc.contributor.alternativeName | 이용준 | - |
dc.contributor.alternativeName | 윤호중 | - |
dc.contributor.alternativeName | 고정헌 | - |
dc.contributor.alternativeName | 이정화 | - |
dc.identifier.bibliographicCitation | Experimental and Molecular Medicine, vol. 37, no. 6, pp. 624-630 | - |
dc.identifier.doi | 10.1038/emm.2005.76 | - |
dc.subject.keyword | BAG3 protein | - |
dc.subject.keyword | Cell differentiation | - |
dc.subject.keyword | Cyclin-dependent kinase inhibitor p27 | - |
dc.subject.keyword | HL-60 cells | - |
dc.subject.keyword | Human | - |
dc.subject.local | BAG3 protein | - |
dc.subject.local | cell differentiation | - |
dc.subject.local | Cell differentiation | - |
dc.subject.local | Cyclin-dependent kinase inhibitor p27 | - |
dc.subject.local | HL-60 cells | - |
dc.subject.local | Human | - |
dc.subject.local | Humans | - |
dc.subject.local | humans | - |
dc.subject.local | human | - |
dc.description.journalClass | Y | - |
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