Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner

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Title
Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner
Author(s)
J P Park; K B Lee; S J Lee; T J Park; Min-Gon Kim; Bong Hyun Chung; Z W Lee; I S Choi; S Y Lee
Bibliographic Citation
Biotechnology and Bioengineering, vol. 92, no. 2, pp. 160-165
Publication Year
2005
Abstract
A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (μCP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fused to the SBD could be specifically immobilized on the micropatterns of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3- hydroxyhexanoate). Laser scanning confocal microscopic studies suggested that two fusion proteins were micropatterned in their functionally active forms. Also, antibody binding assay by surface plasmon resonance suggested that protein-protein interaction studies could be carried out using this system.
Keyword
Microcontact printingPHAPolyhydroxyalkanoatesSubstrate binding domainSurface plasmon resonance
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/bit.20581
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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