TEM sample preparation for cultured neurons on a glass coverslip = Hydrofluoric acid 용액을 이용한 유리 커버글라스에 배양된 신경세포의 전자현미경 시료제작법

Abstract

Cultured neurons from Drosophila brain on a glass coverslip to understand the structural basis of synapse were prepared for TEM observations. Neurons on a coverslip were fixed, dehydrated and embedded in Epon without separating from coverslip. After polymerization, the block was placed in 49% hydrofluoric acid to remove the coverslip. The block was examined under a light microscope to select exact neurons, then trimmed and sectioned for TEM observation.