Identification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration

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dc.contributor.authorYong Sam Kim-
dc.contributor.authorHye Yeon Kang-
dc.contributor.authorJ Y Kim-
dc.contributor.authorS Oh-
dc.contributor.authorC H Kim-
dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorE Miyoshi-
dc.contributor.authorN Taniguchi-
dc.contributor.authorJeong Heon Ko-
dc.date.accessioned2017-04-19T09:04:05Z-
dc.date.available2017-04-19T09:04:05Z-
dc.date.issued2006-
dc.identifier.issn16159853-
dc.identifier.uri10.1002/pmic.200500400ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7288-
dc.description.abstractTo gain a better understanding of the mechanism underlying colon cancer and to search for potential markers of colon cancer prognosis, a comparative proteomic analysis of colon cancer WiDr cells was conducted using 2-DE and lectin blot, followed by identification based on ESI-MS. Through these approaches 14 proteins were identified as candidate target proteins for N-acetylglucosaminyl transferase V (GnT-V) that would be expected to be implicated in the progression of colon cancer. We selected protein tyrosine phosphatase kappa (PTPκ) as a model protein to validate this approach to the discovery of novel biomarkers in colon cancer. PTPκ underwent an aberrant glycosylation in GnT-V-overexpressing WiDr cells, and the aberrantly glycosylated PTPκ was vulnerable to proteolytic cleavage. The enhanced cleavage of PTPκ in GnT-V-overexpressing cells was responsible for the mitigation of the homophilic binding capacity, resulting in an increase in cancer cell migration.-
dc.publisherWiley-
dc.titleIdentification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration-
dc.title.alternativeIdentification of target proteins of N-acetylglucosaminyl transferase V in human colon cancer and implications of protein tyrosine phosphatase kappa in enhanced cancer cell migration-
dc.typeArticle-
dc.citation.titleProteomics-
dc.citation.number4-
dc.citation.endPage1191-
dc.citation.startPage1187-
dc.citation.volume6-
dc.contributor.affiliatedAuthorYong Sam Kim-
dc.contributor.affiliatedAuthorJeong Heon Ko-
dc.contributor.alternativeName김용삼-
dc.contributor.alternativeName강혜연-
dc.contributor.alternativeName김진영-
dc.contributor.alternativeName오세정-
dc.contributor.alternativeName김철호-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeNameMiyoshi-
dc.contributor.alternativeNameTaniguchi-
dc.contributor.alternativeName고정헌-
dc.identifier.bibliographicCitationProteomics, vol. 6, no. 4, pp. 1187-1191-
dc.identifier.doi10.1002/pmic.200500400-
dc.subject.keyword2-DE-
dc.subject.keywordcolon cancer-
dc.subject.keywordglycomics-
dc.subject.keywordN-Acetylglucosaminyl transferase V-
dc.subject.keywordprotein tyrosine phosphatase κ-
dc.subject.local2-DE-
dc.subject.localcolon cancer-
dc.subject.localColon cancer-
dc.subject.localglycomics-
dc.subject.localN-Acetylglucosaminyl transferase V-
dc.subject.localprotein tyrosine phosphatase κ-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Genome Editing Research Center > 1. Journal Articles
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