Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase

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Title
Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase
Author(s)
Choong Yeul Lee; Ki Ho Kim; Sun Yeon Hur; Joo-Hyung Heo; Min Ho Choi; Sang Ki Rhee; Chul Ho Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 16, no. 1, pp. 64-67
Publication Year
2006
Abstract
To enhance the stability of ascorbic acid, the glycosylation of ascorbic acid was studied using the transfructosylation activity of levan fructotransferase. When levan was used as glycosyl donor, a novel fructoside (ascorbic acid 2-fructoside) was formed by the transfructosylation activity of the levan fructotransferase. The production of ascorbic acid 2-fructoside was highly affected by the concentration of the fructosyl acceptor (ascorbic acid). When 35% of ascorbic acid and 2% of levan were incubated with LFTase of 0.5 unit/g-levan at 37°C for 85 h, a maximum 52 g/l of AA-2F was produced.
Keyword
ascorbic acid 2-fructosidefructosideglycosideglycosylationlevan fructotransferase
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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