DC Field | Value | Language |
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dc.contributor.author | Sang Chun Ji | - |
dc.contributor.author | Dockyu Kim | - |
dc.contributor.author | Jung-Hoon Yoon | - |
dc.contributor.author | Tae Kwang Oh | - |
dc.contributor.author | Choong Hwan Lee | - |
dc.date.accessioned | 2017-04-19T09:04:05Z | - |
dc.date.available | 2017-04-19T09:04:05Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7291 | - |
dc.description.abstract | A soil metagenomic library was constructed using an E. coli-fosmid cloning system with environmental DNAs extracted from Kwangreung forest topsoil. We targeted the genes involved in the biosynthesis of bacterial polyketides. Initially, a total of 36 clone pools (10,800 clones) were explored by the PCR-based method using the metagenomic DNAs from each pool and a degenerate primer set, which has been designed based on the highly conserved regions among ketoacyl synthase (KS) domains in actinomycete type I polyketide synthases (PKS Is). Six clone pools were tentatively selected as positive and further examined through a hybridization-based method for selecting a fosmid clone containing PKS I genes. Colony hybridization was performed against fosmid clones from the 6 positive pools, and finally 4 clones were picked out and confirmed to contain the conserved DNA fragment of KS domains. In this study, we present a simple and feasible sorting method for a desired clone from metagenomic libraries. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Sequence-based screening for putative polyketide synthase gene-harboring clones from a soil metagenome library | - |
dc.title.alternative | Sequence-based screening for putative polyketide synthase gene-harboring clones from a soil metagenome library | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 157 | - |
dc.citation.startPage | 153 | - |
dc.citation.volume | 16 | - |
dc.contributor.affiliatedAuthor | Sang Chun Ji | - |
dc.contributor.affiliatedAuthor | Dockyu Kim | - |
dc.contributor.affiliatedAuthor | Jung-Hoon Yoon | - |
dc.contributor.affiliatedAuthor | Tae Kwang Oh | - |
dc.contributor.affiliatedAuthor | Choong Hwan Lee | - |
dc.contributor.alternativeName | 지상천 | - |
dc.contributor.alternativeName | 김덕규 | - |
dc.contributor.alternativeName | 윤정훈 | - |
dc.contributor.alternativeName | 오태광 | - |
dc.contributor.alternativeName | 이충환 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 16, no. 1, pp. 153-157 | - |
dc.subject.keyword | polyketide synthase | - |
dc.subject.keyword | sequence-based screening | - |
dc.subject.keyword | soil metagenome | - |
dc.subject.local | Polyketide synthase | - |
dc.subject.local | polyketide synthase | - |
dc.subject.local | sequence-based screening | - |
dc.subject.local | soil metagenome | - |
dc.subject.local | Soil metagenome | - |
dc.description.journalClass | Y | - |
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