Sequence-based screening for putative polyketide synthase gene-harboring clones from a soil metagenome library

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dc.contributor.authorSang Chun Ji-
dc.contributor.authorDockyu Kim-
dc.contributor.authorJung-Hoon Yoon-
dc.contributor.authorTae Kwang Oh-
dc.contributor.authorChoong Hwan Lee-
dc.date.accessioned2017-04-19T09:04:05Z-
dc.date.available2017-04-19T09:04:05Z-
dc.date.issued2006-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7291-
dc.description.abstractA soil metagenomic library was constructed using an E. coli-fosmid cloning system with environmental DNAs extracted from Kwangreung forest topsoil. We targeted the genes involved in the biosynthesis of bacterial polyketides. Initially, a total of 36 clone pools (10,800 clones) were explored by the PCR-based method using the metagenomic DNAs from each pool and a degenerate primer set, which has been designed based on the highly conserved regions among ketoacyl synthase (KS) domains in actinomycete type I polyketide synthases (PKS Is). Six clone pools were tentatively selected as positive and further examined through a hybridization-based method for selecting a fosmid clone containing PKS I genes. Colony hybridization was performed against fosmid clones from the 6 positive pools, and finally 4 clones were picked out and confirmed to contain the conserved DNA fragment of KS domains. In this study, we present a simple and feasible sorting method for a desired clone from metagenomic libraries.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleSequence-based screening for putative polyketide synthase gene-harboring clones from a soil metagenome library-
dc.title.alternativeSequence-based screening for putative polyketide synthase gene-harboring clones from a soil metagenome library-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage157-
dc.citation.startPage153-
dc.citation.volume16-
dc.contributor.affiliatedAuthorSang Chun Ji-
dc.contributor.affiliatedAuthorDockyu Kim-
dc.contributor.affiliatedAuthorJung-Hoon Yoon-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.affiliatedAuthorChoong Hwan Lee-
dc.contributor.alternativeName지상천-
dc.contributor.alternativeName김덕규-
dc.contributor.alternativeName윤정훈-
dc.contributor.alternativeName오태광-
dc.contributor.alternativeName이충환-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 16, no. 1, pp. 153-157-
dc.subject.keywordpolyketide synthase-
dc.subject.keywordsequence-based screening-
dc.subject.keywordsoil metagenome-
dc.subject.localPolyketide synthase-
dc.subject.localpolyketide synthase-
dc.subject.localsequence-based screening-
dc.subject.localsoil metagenome-
dc.subject.localSoil metagenome-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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