Production of human lactoferrin in transgenic cell suspension cultures of sweet potato

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Title
Production of human lactoferrin in transgenic cell suspension cultures of sweet potato
Author(s)
Sung Ran Min; J W Woo; Won Joong Chung; S K Han; Y B Lee; Jang Ryol Liu
Bibliographic Citation
Biologia Plantarum, vol. 50, no. 1, pp. 131-134
Publication Year
2006
Abstract
Shoot apical meristem-derived calli were transformed with a hLF cDNA in an attempt to produce human lactoferrin (hLF) in transgenic cell suspension cultures of sweet potato [Ipomoea batatas (L.) Lam.]. Calli were bombarded with tungsten particles coated with the binary vector pLSM1 containing a hLF cDNA under the control of the 35S promoter and the neomycin phosphotransferase gene as a selection marker. Calli were then transferred to Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 100 mg dm-3 kanamycin. Kanamycin-resistant calli were selected at four-week intervals and subcultured. Cell suspension cultures were established in liquid MS medium with 4.52 μM 2,4-D. Southern and Northern blot analyses confirmed that hLF cDNA was incorporated into the plant genome and was properly expressed in the cells. ELISA analysis showed that transgenic cells produced hLF up to 3.2 μg mg-1 (total protein).
Keyword
genetic transformationipomoea batatasparticle bombardment
ISSN
0006-3134
Publisher
Acad Sciences Czech Republic, Inst Experimental Botany
DOI
http://dx.doi.org/10.1007/s10535-005-0087-5
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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