Changes in lipoxygenase properties and activity related to postgerminative growth and senescence in oat (Avena sativa L. cv. Victory 1)

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Title
Changes in lipoxygenase properties and activity related to postgerminative growth and senescence in oat (Avena sativa L. cv. Victory 1)
Author(s)
Hoonbok Yi; M A Yi; H T Choi
Bibliographic Citation
Journal of Plant Biology, vol. 48, no. 4, pp. 429-439
Publication Year
2005
Abstract
Lipoxygenase (LOX), one of the main oxidative catalysts in plants, is involved in the regulation of growth and senescence. We investigated changes in LOX activity or its properties as they related to the development of oat plants at four stages (germination, growth, natural senescence, and dark-incubated senescence). LOX activity was high during early growth and at senescence. At pH 4.5, activity showed an abrupt surge compared with a normal enzyme reaction pattern at pH 6.5. The optimum reaction temperature was 25°C; LOX and peroxidase exhibited similar activity patterns. Polyacrylamide gel electrophoresis revealed that the purified LOX consisted of three isoenzymes in germinating seeds, two in growing seedlings, and three during both natural and dark-induced senescence. As determined by isoelectric focusing, the isoelectric points (pl) of LOX ranged from 3.6 to 6.5 throughout the four developmental stages; for natural or dark-induced senescence, the pl was 9.0.
Keyword
isoelectric focusinglipoxygenaseoatspolyacrylamide gel electrophoresisseedlingsseeds
ISSN
I000-0094
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF03030585
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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