Regulation of pho regulon gene expression by the carbon control protein A, CcpA, in Bacillus subtilis

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Title
Regulation of pho regulon gene expression by the carbon control protein A, CcpA, in Bacillus subtilis
Author(s)
Soo Keun Choi; M H Saier
Bibliographic Citation
Journal of Molecular Microbiology and Biotechnology, vol. 10, no. 1, pp. 40-50
Publication Year
2005
Abstract
Bacterial regulons involved in carbon, nitrogen and phosphorus metabolism must interact for purposes of coordination, but the mechanisms involved are not understood. We here report that the carbon control protein-A (CcpA) of Bacillus subtilis, primarily concerned with carbon metabolism, influences expression of various phosphorus (pho) regulon genes including the two alkaline phosphatase structural genes, phoA and phoB. The directions and magnitudes of the effects of glucose and the loss of CcpA on these two genes depend on growth conditions, but they always correlate inversely. Absolute expression levels of phoA and phoB depend on a rich nitrogen source, and gene activation by a fermentable substrate such as glucose depends on the presence of a respiratory substrate such as succinate. We show that these CcpA-dependent glucose effects can be explained by the effects of glucose and CcpA acting on the phoPR operon. Although a good CcpA-binding site (CRE) is found in the control region of the phoPR operon, direct regulation of phoPR gene expression by CcpA via this CRE could not account for the effects of glucose and CcpA on phoA and phoB gene expression. We conclude that CcpA exerts indirect control over the pho regulon by a mechanism that involves CcpA and PhoRP but does not involve the phoPR operon CRE.
Keyword
carbon catabolite regulationCcpApho regulonphosphate metabolismtranscription
ISSN
1464-1801
Publisher
Karger
DOI
http://dx.doi.org/10.1159/000090347
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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