Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis

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Title
Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis
Author(s)
Yong Mahn Han; Seok Ho Kim; Yong-Kook Kang
Bibliographic Citation
Methods in Molecular Biology, vol. 325, pp. 251-260
Publication Year
2005
Abstract
For developmental competence of mammalian embryos, dynamic epigenetic changes to both the maternally and paternally derived contributions to the genome of the zygote should be brought about in the early cleavage stages. DNA methylation is a typical epigenetic mark modified during pre-implantation development. Here, we describe how to analyze DNA methylation profiles in early-stage embryos.
Keyword
primer DNADNA methylationmutagenesisDNA methylationDNA primersDNA restriction enzymesmutagenesis
ISSN
1064-3745
Publisher
Humana Press
DOI
http://dx.doi.org/10.1385/1-59745-005-7:251
Type
Article
Appears in Collections:
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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