Improved L-threonine production of Escherichia coli mutant by optimization of culture conditions

Cited 35 time in scopus
Metadata Downloads
Title
Improved L-threonine production of Escherichia coli mutant by optimization of culture conditions
Author(s)
M-H Lee; Hong-Weon Lee; J-H Park; Jungoh Ahn; Joon-Ki Jung; Y-I Hwang
Bibliographic Citation
Journal of Bioscience and Bioengineering, vol. 101, no. 2, pp. 127-130
Publication Year
2006
Abstract
l-Threonine production was investigated in a minimal salt medium using l-threonine-overproducing Escherichia coli MT201, derived from E. coli K-12. It was observed that dry cell weight reached 12.5 g/l with 15.9 g/l l-threonine. To increase dry cell weight and l-threonine production, the fermentation process was optimized. When biotin was added as growth factor, l-threonine production reached 52.0 g/l from 15.9 g/l without biotin. Dry cell weight and l-threonine production were further increased by continuous feeding of the feed media with an optimized l-methionine concentration (5.0 g/l). However, high-cell-density culture caused oxygen-limited condition, which resulted in the accumulation of organic acids. To overcome this problem, oxygen-enriched air was supplied to the fermentor with the minimal salt medium. Under these optimal conditions, we achieved an l-threonine production of 80.2 g/l in the minimal salt medium.
Keyword
biotinEscherichia coli MT201l-methioninel-threonineminimal salt medium
ISSN
1389-1723
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1263/jbb.101.127
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.