DC Field | Value | Language |
---|---|---|
dc.contributor.author | Su-Young Oh | - |
dc.contributor.author | Jun Hee Lee | - |
dc.contributor.author | Jung-Eun Gil | - |
dc.contributor.author | J H Kim | - |
dc.contributor.author | Young Il Yeom | - |
dc.contributor.author | Eun Yi Moon | - |
dc.date.accessioned | 2017-04-19T09:04:23Z | - |
dc.date.available | 2017-04-19T09:04:23Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0014-4827 | - |
dc.identifier.uri | 10.1016/j.yexcr.2006.01.030 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7387 | - |
dc.description.abstract | The development of paclitaxel-resistance in tumors is one of the most significant obstacles to successful therapy. Thymosin-beta-4 (TB4) has been known as actin-sequestering protein and functions in tumor metastasis. Here, we overexpressed TB4 in HeLa cells (TB4-HeLa) and examined the effect of TB4 in paclitaxel-induced cell death. TB4-HeLa cells showed a higher growth rate and a lower percentage of basal apoptosis than HeLa cells. TB4-HeLa cells were more resistant to paclitaxel-induced cell death than HeLa cells. TB4 transcript expression with paclitaxel treatment was dose-dependently increased in HeLa cells but that was not in TB4-HeLa cells. Small interfering RNA (siRNA) of TB4 inhibited HeLa cell growth and enhanced paclitaxel-induced cell death. Basal ERK phosphorylation was elevated and basal p38 kinase phosphorylation was reduced in paclitaxel non-treated TB4-HeLa cells. When treated with paclitaxel, cell death and resistance-induction were independent of ERK and p38 kinase activation. Paclitaxel-resistance of TB4-HeLa cells was overcome by the inhibition of basal ERK activity with PD98059 pre-treatment. The inhibition of basal p38 kinase activity with SB203580 pre-treatment attenuated the paclitaxel-induced HeLa cell death. In conclusion, TB4 induced paclitaxel-resistance through the elevation of basal level of ERK phosphorylation. Therefore, TB4 could be a novel target to regulate paclitaxel-resistance. | - |
dc.publisher | Elsevier | - |
dc.title | ERK activation by Thymosin-beta-4 (TB4) overexpression induces paclitaxel-resistance | - |
dc.title.alternative | ERK activation by Thymosin-beta-4 (TB4) overexpression induces paclitaxel-resistance | - |
dc.type | Article | - |
dc.citation.title | Experimental Cell Research | - |
dc.citation.number | 9 | - |
dc.citation.endPage | 1657 | - |
dc.citation.startPage | 1651 | - |
dc.citation.volume | 312 | - |
dc.contributor.affiliatedAuthor | Su-Young Oh | - |
dc.contributor.affiliatedAuthor | Jun Hee Lee | - |
dc.contributor.affiliatedAuthor | Jung-Eun Gil | - |
dc.contributor.affiliatedAuthor | Young Il Yeom | - |
dc.contributor.affiliatedAuthor | Eun Yi Moon | - |
dc.contributor.alternativeName | 오수영 | - |
dc.contributor.alternativeName | 이준희 | - |
dc.contributor.alternativeName | 길정은 | - |
dc.contributor.alternativeName | 김정희 | - |
dc.contributor.alternativeName | 염영일 | - |
dc.contributor.alternativeName | 문은이 | - |
dc.identifier.bibliographicCitation | Experimental Cell Research, vol. 312, no. 9, pp. 1651-1657 | - |
dc.identifier.doi | 10.1016/j.yexcr.2006.01.030 | - |
dc.subject.keyword | ERK | - |
dc.subject.keyword | HeLa cell | - |
dc.subject.keyword | Paclitaxel | - |
dc.subject.keyword | Resistance | - |
dc.subject.keyword | Thymosin-beta-4 | - |
dc.subject.local | ERK | - |
dc.subject.local | Erk | - |
dc.subject.local | HeLa cell | - |
dc.subject.local | HeLa cells | - |
dc.subject.local | Hela cell | - |
dc.subject.local | paclitaxel | - |
dc.subject.local | Paclitaxel | - |
dc.subject.local | Resistance | - |
dc.subject.local | resistance | - |
dc.subject.local | Thymosin β4 | - |
dc.subject.local | Thymosin-beta-4 (TB4) | - |
dc.subject.local | Thymosin beta-4 | - |
dc.subject.local | Thymosin-beta-4 | - |
dc.subject.local | thymosin beta-4 | - |
dc.description.journalClass | Y | - |
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