Suppression of pepper SGT1 and SKP1 causes severe retardation of plant growth and compromises basal resistance

Cited 11 time in scopus
Metadata Downloads
Title
Suppression of pepper SGT1 and SKP1 causes severe retardation of plant growth and compromises basal resistance
Author(s)
Eunsook Chung; Choong-Min Ryu; S K Oh; R N Kim; Jeong Mee ParkHye Sun Cho; Sanghyeob Lee; Jae Sun Moon; Seung Hwan Park; Doil Choi
Bibliographic Citation
Physiologia Plantarum, vol. 126, no. 4, pp. 605-617
Publication Year
2006
Abstract
SGT1 associates with suppressor of kinetochore protein (Skp1)-Cullin-F-box (SCF)-ubiquitin-ligase complexes playing important roles in controlling developmental processes and defense responses in plants, yeast, and human. In this study, full-length cDNAs of Sgt1 and Skp1 orthologues were isolated from pepper (Capsicum annuum L.) to characterize their functions. Protein sequences of CaSgt1 and CaSkp1 showed high degrees of similarities with their homologues in other plant species. Southern blot analyses revealed that CaSgt1 was a single copy gene in the pepper genome, whereas CaSkp1 corresponded to multi copy genes. Levels of CaSgt1 and CaSkp1 mRNAs increased in pepper leaves in response to incompatible pathogen challenge or salicylic acid treatment. Silencing of CaSgt1 or CaSkp1 using Tobacco rattle virus-based virus-induced gene-silencing (VIGS) system resulted in severe dwarfism and final damping-off symptom in the greenhouse. To identify factors determining damping-off symptom in CaSgt1- or CaSkp1-silenced plants, we employed VIGS under sterile conditions. Under such conditions, damping-off symptom was not observed suggesting that CaSgt1 and CaSkp1 play an essential role in plant growth and development as well as basal disease resistance in pepper plant.
ISSN
0031-9317
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1399-3054.2006.00631.x
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.