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- Suppression of pepper SGT1 and SKP1 causes severe retardation of plant growth and compromises basal resistance
- Eunsook Chung; Choong-Min Ryu; S K Oh; R N Kim; Jeong Mee Park; Hye Sun Cho; Sanghyeob Lee; Jae Sun Moon; Seung Hwan Park; Doil Choi
- Bibliographic Citation
- Physiologia Plantarum, vol. 126, no. 4, pp. 605-617
- Publication Year
- SGT1 associates with suppressor of kinetochore protein (Skp1)-Cullin-F-box (SCF)-ubiquitin-ligase complexes playing important roles in controlling developmental processes and defense responses in plants, yeast, and human. In this study, full-length cDNAs of Sgt1 and Skp1 orthologues were isolated from pepper (Capsicum annuum L.) to characterize their functions. Protein sequences of CaSgt1 and CaSkp1 showed high degrees of similarities with their homologues in other plant species. Southern blot analyses revealed that CaSgt1 was a single copy gene in the pepper genome, whereas CaSkp1 corresponded to multi copy genes. Levels of CaSgt1 and CaSkp1 mRNAs increased in pepper leaves in response to incompatible pathogen challenge or salicylic acid treatment. Silencing of CaSgt1 or CaSkp1 using Tobacco rattle virus-based virus-induced gene-silencing (VIGS) system resulted in severe dwarfism and final damping-off symptom in the greenhouse. To identify factors determining damping-off symptom in CaSgt1- or CaSkp1-silenced plants, we employed VIGS under sterile conditions. Under such conditions, damping-off symptom was not observed suggesting that CaSgt1 and CaSkp1 play an essential role in plant growth and development as well as basal disease resistance in pepper plant.
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- Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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