Cloning of superoxide dismutase (SOD) gene of lily 'Marcopolo' and expression in transgenic potatoes

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Title
Cloning of superoxide dismutase (SOD) gene of lily 'Marcopolo' and expression in transgenic potatoes
Author(s)
J Y Park; Hyun Soon Kim; Jung-Won Youm; Mi-Sun Kim; K S Kim; Hyouk Joung; Jae Heung Jeon
Bibliographic Citation
Journal of Applied Biological Chemistry, vol. 49, no. 1, pp. 1-7
Publication Year
2006
Abstract
Differential display reverse transcription PCR (DDRT-PCR) analysis was performed on lily 'Marcopolo' bulb scale for isolation of expressed genes during bulblet formation. Cu/Zn lily-superoxide dismutase (LSOD) of 872 bp gene, with ability to scavenge reactive oxygen in stress environment, was isolated. Northern blot analysis showed expression levels of LSOD maximized 12 days after bulblet formation. Ti plasmid vectors were constructed with sense and antisense expressions of LSOD gene and transformed into potato. Southern blot analysis of transgenic potatoes revealed different copies of T-DNA were incorporated into potato genome.
Keyword
tuberizationtransgenic potatolily bulblet formationsuperoxide dismutase
ISSN
1229-2737
Publisher
Springer
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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