Assessment of inactivation for Salmonella spp. on chicken meat using confocal laser microscopy and flow cytometry = 공초점 현미경 및 유세포 분류기를 이용한 계육에서의 Salmonella균 불활성화 평가

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Title
Assessment of inactivation for Salmonella spp. on chicken meat using confocal laser microscopy and flow cytometry = 공초점 현미경 및 유세포 분류기를 이용한 계육에서의 Salmonella균 불활성화 평가
Author(s)
K I Jang; D H Chung; S D Ha; K S Kim; K H Lee; Min-Gon Kim; C H Kim; K Y Kim
Bibliographic Citation
Korean Journal of Food Science & Technology, vol. 38, no. 2, pp. 290-294
Publication Year
2006
Abstract
Inactivation rates of Salmonella enteritidis in vitro and in vivo were assessed using confocal microscopy and flow cytometry. S. enteritidis was inactivated with 1% (w/v) trisodium phosphate (TSP) and live cells, and inactive cells were distinguished by staining with fluorescent probe, LIVE/DEAD BacLight Bacteria Viability stain. After TSP treatment for 1 min, most of Salmonella cells changed from green (live cells) fluorescence to red (inactive cells) fluorescence, indication of effective sanitizing. Inactivation efficiency and contamination sites of S. enteritidis on chicken skin by TSP treatment were assessed using confocal laser microscopy. Precise flow cytometry histograms for viability changes of S. enteritidis. after TSP treatments were obtained. Efficiency of various sanitizer treatments on foodborne pathogens could be assessed using this method.
Keyword
confocal microscopyflow cytometryassessmenttrisodium phosphatesalmonella enteritidis
ISSN
0367-6293
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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