DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jin Young Jeong | - |
dc.contributor.author | Tai-Hwan Ha | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.date.accessioned | 2017-04-19T09:04:29Z | - |
dc.date.available | 2017-04-19T09:04:29Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | 10.1016/j.aca.2006.03.102 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7416 | - |
dc.description.abstract | Gold-coated magnetic nanoparticle (GMP) was manufactured and used as a carrier for the immobilization of hexa-arginine-tagged esterase (Arg6-esterase). The formation of gold shell on the magnetic nanoparticle (Fe2O3) was performed by an iterative reduction method using hydroxylamine as a reductant. The surface of the GMP was further functionalized with mercapto-hexadecanoic acid (MHA) to tether the positively charged Arg6-esterase effectively. The enzymatic activity of the immobilized Arg6-esterase was investigated by monitoring the dissociation rate of its colorimetric substrate, p-nitrophenol butyrate (pNPB). Although the immobilized enzyme exhibited a lower activity (∼60%) compared to the free one, its original activity was found to be retained even after seven times repetitive uses by magnetic decantation. | - |
dc.publisher | Elsevier | - |
dc.title | Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles | - |
dc.title.alternative | Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles | - |
dc.type | Article | - |
dc.citation.title | Analytica Chimica Acta | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 209 | - |
dc.citation.startPage | 203 | - |
dc.citation.volume | 569 | - |
dc.contributor.affiliatedAuthor | Jin Young Jeong | - |
dc.contributor.affiliatedAuthor | Tai-Hwan Ha | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 정진영 | - |
dc.contributor.alternativeName | 하태환 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.identifier.bibliographicCitation | Analytica Chimica Acta, vol. 569, pp. 203-209 | - |
dc.identifier.doi | 10.1016/j.aca.2006.03.102 | - |
dc.subject.keyword | Enzyme immobilization | - |
dc.subject.keyword | Gold-coated magnetic nanoparticle (GMP) | - |
dc.subject.keyword | Hexa-arginine-tagged esterase (Arg6-esterase) | - |
dc.subject.keyword | Magnetic separation | - |
dc.subject.local | enzyme immobilization | - |
dc.subject.local | Enzyme immobilization | - |
dc.subject.local | Gold-coated magnetic nanoparticle (GMP) | - |
dc.subject.local | Hexa-arginine-tagged esterase (Arg6-esterase) | - |
dc.subject.local | Magnetic separation | - |
dc.description.journalClass | Y | - |
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