Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles

Cited 60 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJin Young Jeong-
dc.contributor.authorTai-Hwan Ha-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:04:29Z-
dc.date.available2017-04-19T09:04:29Z-
dc.date.issued2006-
dc.identifier.issn0003-2670-
dc.identifier.uri10.1016/j.aca.2006.03.102ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7416-
dc.description.abstractGold-coated magnetic nanoparticle (GMP) was manufactured and used as a carrier for the immobilization of hexa-arginine-tagged esterase (Arg6-esterase). The formation of gold shell on the magnetic nanoparticle (Fe2O3) was performed by an iterative reduction method using hydroxylamine as a reductant. The surface of the GMP was further functionalized with mercapto-hexadecanoic acid (MHA) to tether the positively charged Arg6-esterase effectively. The enzymatic activity of the immobilized Arg6-esterase was investigated by monitoring the dissociation rate of its colorimetric substrate, p-nitrophenol butyrate (pNPB). Although the immobilized enzyme exhibited a lower activity (∼60%) compared to the free one, its original activity was found to be retained even after seven times repetitive uses by magnetic decantation.-
dc.publisherElsevier-
dc.titleEnhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles-
dc.title.alternativeEnhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles-
dc.typeArticle-
dc.citation.titleAnalytica Chimica Acta-
dc.citation.number0-
dc.citation.endPage209-
dc.citation.startPage203-
dc.citation.volume569-
dc.contributor.affiliatedAuthorJin Young Jeong-
dc.contributor.affiliatedAuthorTai-Hwan Ha-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName정진영-
dc.contributor.alternativeName하태환-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationAnalytica Chimica Acta, vol. 569, pp. 203-209-
dc.identifier.doi10.1016/j.aca.2006.03.102-
dc.subject.keywordEnzyme immobilization-
dc.subject.keywordGold-coated magnetic nanoparticle (GMP)-
dc.subject.keywordHexa-arginine-tagged esterase (Arg6-esterase)-
dc.subject.keywordMagnetic separation-
dc.subject.localenzyme immobilization-
dc.subject.localEnzyme immobilization-
dc.subject.localGold-coated magnetic nanoparticle (GMP)-
dc.subject.localHexa-arginine-tagged esterase (Arg6-esterase)-
dc.subject.localMagnetic separation-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.