AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants

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Title
AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants
Author(s)
C H Kang; W Y Jung; Y H Kang; J Y Kim; D G Kim; Jae Cheol Jeong; D W Baek; J B Jin; Jiyoung Lee; M O Kim; W S Chung; T Mengiste; H Koiwa; Sang Soo Kwak; J D Bahk; S Y Lee; J S Nam; D J Yun; M J Cho
Bibliographic Citation
Cell Death and Differentiation, vol. 13, no. 1, pp. 84-95
Publication Year
2006
Abstract
Calmodulin (CaM) influences many cellular processes by interacting with various proteins. Here, we isolated AtBAG6, an Arabidopsis CaM-binding protein that contains a central BCL-2-associated athanogene (BAG) domain. In yeast and plants, overexpression of AtBAG6 induced cell death phenotypes consistent with programmed cell death (PCD). Recombinant AtBAG6 had higher affinity for CaM in the absence of free Ca2+ than in its presence. An IQ motif (IQXXXRGXXXR, where X denotes any amino-acid) was required for Ca2+-independent CaM complex formation and single amino-acid changes within this motif abrogated both AtBAG6-activated CaM-binding and cell death in yeast and plants. A 134-amino-acid stretch, encompassing both the IQ motif and BAG domain, was sufficient to induce cell death. Agents generating oxygen radicals, which are known to be involved in plant PCD, specifically induced the AtBAG6 transcript. Collectively, these results suggest that AtBAG6 is a stress-upregulated CaM-binding protein involved in plant PCD.
Keyword
ArabidopsisBAG domainCalciumCalmodulinCaM-binding proteinIQ motifStress
ISSN
1350-9047
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/sj.cdd.4401712
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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