Protein composition of domestic and glyphosate-tolerant soybean = 국내산 및 제초제 내성 콩의 단백질 조성 분석

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Title
Protein composition of domestic and glyphosate-tolerant soybean = 국내산 및 제초제 내성 콩의 단백질 조성 분석
Author(s)
C H Wei; D E Sok; Y H Yang; S H Oh; Hyoung-Chin KimWoon Kee Yoon; Hwan Mook Kim; M R Kim
Bibliographic Citation
Journal of Korean Society for Food Science & Nutrition, vol. 35, no. 4, pp. 470-475
Publication Year
2006
Abstract
In order to elucidate the differences of protein profiles among soybean cultivars, the protein composition of three conventional domestic soybean cultivars and two imported ones including glyphosate.tolerant HS2906 was analyzed by total nitrogen measurement, amino acid analysis and PAGE/densitometry. There were no statistically significant differences in the levels of any amino acid, including aromatic amino acids, between glyphosate.tolerant soybean and the conventional soybean WS82. In the extraction of protein, the SDS/buffer system was more efficient than the defatting/water system. The SDS.PAGE/densitometry analysis showed that there was a similar profile of proteins among cultivars, although the amount of total protein ranged from 380.2 mg/g to 423.9 mg/g. In addition, there was no discernable difference of protein profile between glyphosate. tolerant soybean (total protein amount, 380.2 mg/g) and the conventional soybean WS82 (390.2 mg/g), although the amount of β.conglycinin (55 kDa) was lower in glyphosate.tolerant soybean. Meanwhile, the amount of 25 kDa protein was greater in domestic soybean cultivars than imported ones. Thus, normal PAGE/ densitometry method would be useful to analyze the difference in protein profiles of soybean proteins, and furthermore evaluate the protein profile of proteins between GMO and conventional soybean.
Keyword
protein profilesoybeanPAGE
ISSN
I000-0088
Publisher
South Korea
DOI
http://dx.doi.org/10.3746/jkfn.2006.35.4.470
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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