Polyhydroxyalkanoate chip for the specific immobilization of recombinant proteins and its applications in immunodiagnostics

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Title
Polyhydroxyalkanoate chip for the specific immobilization of recombinant proteins and its applications in immunodiagnostics
Author(s)
T J Park; J P Park; S J Lee; Hyo Jeong Hong; S Y Lee
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 11, no. 2, pp. 173-177
Publication Year
2006
Abstract
In this study, a novel strategy was developed for the highly selective immobilization of proteins, using the polyhydroxyalkanoate (PHA) depolymerase substrate binding domain (SBD) as an active binding domain. In order to determine the appropriacy of this method for immunodiagnostic assays, the single-chain antibody (ScFv) against the hepatitis B virus (HBV) preS2 surface protein and the severe acute respiratory syndrome coronavirus (SARS-CoV) envelope protein (SCVe) were fused to the SBD, then directly immobilized on PHA-coated slides via microspotting. The fluorescence-labeled HBV antigen and the antibody against SCVe were then utilized to examine specific interactions on the PHA-coated surfaces. Fluorescence signals were detected only at the spotted positions, thereby indicating a high degree of affinity and selectivity for their corresponding antigens/antibodies. Furthermore, we detected small amounts of ScFv-SBD (2.7 ng/mL) and SCVe-SBD fusion proteins (0.6 ng/mL). Therefore, this microarray platform technology, using PHA and SBD, appears generally appropriate for immunodiagnosis, with no special requirements with regard to synthetic or chemical modification of the biomolecules or the solid surface.
Keyword
HBV preS2 surface proteinMicroarrayP(3HB) depolymerase substrate binding domainPoly(3-hydroxybutyrate)SARS-CoV envelope protein
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF02931904
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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