Over-production of β-carotene from metabolically engineered Escherichia coli

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Title
Over-production of β-carotene from metabolically engineered Escherichia coli
Author(s)
S W Kim; J B Kim; W H Jung; J H Kim; Joon-Ki Jung
Bibliographic Citation
Biotechnology Letters, vol. 28, no. 12, pp. 897-904
Publication Year
2006
Abstract
To produce recombinant β-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. β-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient β-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal β-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l-1 and 240 mg l-1, respectively, with overall productivities of 7.8 mg l -1 h-1 and 4.8 mg l-1 h-1.
Keyword
β-CaroteneMetabolic engineeringOver-productionSynthetic operon
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-006-9023-9
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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