Preparation of soluble silk peptides by food-grade proteinases = 효소분해에 의한 가용성 실크 펩타이드의 제조

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dc.contributor.authorJae Seok Ha-
dc.contributor.authorJae Jun Song-
dc.contributor.authorH K Cho-
dc.contributor.authorSeung Goo Lee-
dc.date.accessioned2017-04-19T09:04:45Z-
dc.date.available2017-04-19T09:04:45Z-
dc.date.issued2006-
dc.identifier.issn0257-2389-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7464-
dc.description.abstractEnzymatic hydrolysis of silk fibers were investigated for the preparation of soluble silk peptides by ten food-grade proteases from Bacillus, Aspergillus, and plant sources. Silk fibers were dissolved for 1 hr in a 2:1 cosolvent (50% CaCl2: ethanol) by heating at 90°C. The silk solution was filtered to remove impurity particles and desalted for 50 hours by a dialysis process to remove the used cosolvent. When the silk hydrolysis was performed at 45°C for 2 hours, most proteases from Bacillus and Aspergillus generated large amounts of insoluble aggregates. On the contrary, proteases from plant sources produced much less aggregates during prolonged incubations and also exhibited high hydrolysis activities. In regards of the solubility and broad molecular sizes of produced silk peptides, Bromelain was finally selected and applied for the enzymatic hydrolysis of silk fibers.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titlePreparation of soluble silk peptides by food-grade proteinases = 효소분해에 의한 가용성 실크 펩타이드의 제조-
dc.title.alternativePreparation of soluble silk peptides by food-grade proteinases-
dc.typeArticle-
dc.citation.titleKorean Journal of (Applied) Microbiology & Biotechnology-
dc.citation.number2-
dc.citation.endPage120-
dc.citation.startPage115-
dc.citation.volume34-
dc.contributor.affiliatedAuthorJae Seok Ha-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.alternativeName하재석-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName조형권-
dc.contributor.alternativeName이승구-
dc.identifier.bibliographicCitationKorean Journal of (Applied) Microbiology & Biotechnology, vol. 34, no. 2, pp. 115-120-
dc.subject.keywordfood-grade protease-
dc.subject.keywordsilk fibroin-
dc.subject.keywordsoluble silk peptide-
dc.subject.keywordsilk-
dc.subject.localfood-grade protease-
dc.subject.localsilk fibroin-
dc.subject.localsoluble silk peptide-
dc.subject.localsilk-
dc.description.journalClassN-
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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