DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jae Seok Ha | - |
dc.contributor.author | Jae Jun Song | - |
dc.contributor.author | H K Cho | - |
dc.contributor.author | Seung Goo Lee | - |
dc.date.accessioned | 2017-04-19T09:04:45Z | - |
dc.date.available | 2017-04-19T09:04:45Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0257-2389 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/7464 | - |
dc.description.abstract | Enzymatic hydrolysis of silk fibers were investigated for the preparation of soluble silk peptides by ten food-grade proteases from Bacillus, Aspergillus, and plant sources. Silk fibers were dissolved for 1 hr in a 2:1 cosolvent (50% CaCl2: ethanol) by heating at 90°C. The silk solution was filtered to remove impurity particles and desalted for 50 hours by a dialysis process to remove the used cosolvent. When the silk hydrolysis was performed at 45°C for 2 hours, most proteases from Bacillus and Aspergillus generated large amounts of insoluble aggregates. On the contrary, proteases from plant sources produced much less aggregates during prolonged incubations and also exhibited high hydrolysis activities. In regards of the solubility and broad molecular sizes of produced silk peptides, Bromelain was finally selected and applied for the enzymatic hydrolysis of silk fibers. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Preparation of soluble silk peptides by food-grade proteinases = 효소분해에 의한 가용성 실크 펩타이드의 제조 | - |
dc.title.alternative | Preparation of soluble silk peptides by food-grade proteinases | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of (Applied) Microbiology & Biotechnology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 120 | - |
dc.citation.startPage | 115 | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Jae Seok Ha | - |
dc.contributor.affiliatedAuthor | Jae Jun Song | - |
dc.contributor.affiliatedAuthor | Seung Goo Lee | - |
dc.contributor.alternativeName | 하재석 | - |
dc.contributor.alternativeName | 송재준 | - |
dc.contributor.alternativeName | 조형권 | - |
dc.contributor.alternativeName | 이승구 | - |
dc.identifier.bibliographicCitation | Korean Journal of (Applied) Microbiology & Biotechnology, vol. 34, no. 2, pp. 115-120 | - |
dc.subject.keyword | food-grade protease | - |
dc.subject.keyword | silk fibroin | - |
dc.subject.keyword | soluble silk peptide | - |
dc.subject.keyword | silk | - |
dc.subject.local | food-grade protease | - |
dc.subject.local | silk fibroin | - |
dc.subject.local | soluble silk peptide | - |
dc.subject.local | silk | - |
dc.description.journalClass | N | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.