Identification of novel genes with proapoptotic activity

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Title
Identification of novel genes with proapoptotic activity
Author(s)
Eun Ju Kang; J M Kim; N Kim; Kyung Mi Park; Seong-Min Park; Nam-Soon Kim; Hyang Sook Yoo; Young Il Yeom; Soo Jung Kim
Bibliographic Citation
Genomics & Informatics, vol. 4, no. 2, pp. 77-79
Publication Year
2006
Abstract
In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypothetical genes with proapoptotic activity.
Keyword
novel proapoptotic genesapoptosisapoptotic nucleifluorescence microscope
ISSN
I000-0158
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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