Homogeneous versus heterogeneous probes for microbial ecological microarrays

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Title
Homogeneous versus heterogeneous probes for microbial ecological microarrays
Author(s)
Jin-Woo Bae; Yong Ha Park
Bibliographic Citation
Trends in Biotechnology, vol. 24, no. 7, pp. 318-323
Publication Year
2006
Abstract
Microbial ecological microarrays have been developed for investigating the composition and functions of microorganism communities in environmental niches. These arrays include microbial identification microarrays, which use oligonucleotides, gene fragments or microbial genomes as probes. In this article, the advantages and disadvantages of each type of probe are reviewed. Oligonucleotide probes are currently useful for probing uncultivated bacteria that are not amenable to gene fragment probing, whereas the functional gene fragments amplified randomly from microbial genomes require phylogenetic and hierarchical categorization before use as microbial identification probes, despite their high resolution for both specificity and sensitivity. Until more bacteria are sequenced and gene fragment probes are thoroughly validated, heterogeneous bacterial genome probes will provide a simple, sensitive and quantitative tool for exploring the ecosystem structure.
ISSN
0167-7799
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.tibtech.2006.04.010
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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