Proteomic analysis and the antimetastatic effect of N-(4-methyl)phenyl-O-(4-methoxy) phenyl-thionocarbamate-induced apoptosis in human melanoma SK-MEL-28 cells

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dc.contributor.authorS L Choi-
dc.contributor.authorY S Choi-
dc.contributor.authorY K Kim-
dc.contributor.authorN D Sung-
dc.contributor.authorChang-Won Kho-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorEun-Mi Kim-
dc.contributor.authorJeong-Hyung Lee-
dc.contributor.authorK M Kim-
dc.contributor.authorM Y Kim-
dc.contributor.authorP K Myung-
dc.date.accessioned2017-04-19T09:04:48Z-
dc.date.available2017-04-19T09:04:48Z-
dc.date.issued2006-
dc.identifier.issn0253-6269-
dc.identifier.uri10.1007/BF02969398ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7477-
dc.description.abstractWe employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-κB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.-
dc.publisherPharmaceutical Soc Korea-
dc.titleProteomic analysis and the antimetastatic effect of N-(4-methyl)phenyl-O-(4-methoxy) phenyl-thionocarbamate-induced apoptosis in human melanoma SK-MEL-28 cells-
dc.title.alternativeProteomic analysis and the antimetastatic effect of N-(4-methyl)phenyl-O-(4-methoxy) phenyl-thionocarbarmate-induced apoptosis in human melanoma SK-MEL-28 cells-
dc.typeArticle-
dc.citation.titleArchives of Pharmacal Research-
dc.citation.number3-
dc.citation.endPage234-
dc.citation.startPage224-
dc.citation.volume29-
dc.contributor.affiliatedAuthorChang-Won Kho-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorEun-Mi Kim-
dc.contributor.affiliatedAuthorJeong-Hyung Lee-
dc.contributor.alternativeName최수라-
dc.contributor.alternativeName최윤실-
dc.contributor.alternativeName김영관-
dc.contributor.alternativeName성낙도-
dc.contributor.alternativeName고창원-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName김은미-
dc.contributor.alternativeName이정형-
dc.contributor.alternativeName김경미-
dc.contributor.alternativeName김민영-
dc.contributor.alternativeName명평근-
dc.identifier.bibliographicCitationArchives of Pharmacal Research, vol. 29, no. 3, pp. 224-234-
dc.identifier.doi10.1007/BF02969398-
dc.subject.keywordapoptosis-
dc.subject.keywordmetastasis-
dc.subject.keywordN-(4-methyl)phenyl-O-(4-methoxy)phenyl- thionocarbamate-
dc.subject.keywordproteome map-
dc.subject.keywordSK-MEL-26 cells-
dc.subject.localapoptosis-
dc.subject.localApoptosis-
dc.subject.localmetastasis-
dc.subject.localMetastasis-
dc.subject.localN-(4-methyl)phenyl-O-(4-methoxy)phenyl- thionocarbamate-
dc.subject.localproteome map-
dc.subject.localSK-MEL-26 cells-
dc.description.journalClassY-
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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