Oral administration of human papillomavirus type 16 E7 displayed on Lactobacillus casei induces E7-specific antitumor effects in C57/BL6 mice = 파필로마바이러스 유산균 치료백신 개발

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Title
Oral administration of human papillomavirus type 16 E7 displayed on Lactobacillus casei induces E7-specific antitumor effects in C57/BL6 mice = 파필로마바이러스 유산균 치료백신 개발
Author(s)
Haryoung Poo; H M Pyo; T Y Lee; Sun Woo Yoon; J S Lee; C J Kim; M H Sung; S H Lee
Bibliographic Citation
International Journal of Cancer, vol. 119, no. 7, pp. 1702-1709
Publication Year
2006
Abstract
The mounting of a specific immune response against the human papillomavirus type 16 E7 protein (HPV16 E7) is important for eradication of HPV16 E7-expressing cancer cells from the cervical mucosa. To induce a mucosal immune response by oral delivery of the E7 antigen, we expressed the HPV16 E7 antigen on the surface of Lactobacillus casei by employing a novel display system in which the poly-γ-glutamic acid (γ-PGA) synthetase complex A (PgsA) from Bacillus subtilis (chungkookjang) was used as an anchoring motif. After surface expression of the HPV16 E7 protein was confirmed by Western blot, flow cytometry and immunofluorescence microscopy, mice were orally inoculated with L. casei-PgsA-E7. E7-specific serum IgG and mucosal IgA productions were enhanced after oral administration and significantly enhanced after boosting. Systemic and local cellular immunities were significantly increased after boosting, as shown by increased counts of lymphocytes (SI = 9.7 ± 1.8) and IFN-γ secreting cells [510 ± 86 spot-forming cells/106 cells] among splenocytes and increased IFN-γ in supernatants of vaginal lymphocytes. Furthermore, in an E7-based mouse tumor model, animals receiving orally administered L. casei-PgsA-E7 showed reduced tumor size and increased survival rate versus mice receiving control (L. casei-PgsA) immunization. These results collectively indicate that the oral administration of E7 displayed on lactobacillus induces cellular immunity and antitumor effects in mice.
Keyword
Antitumor effectCellular immunityHPV16 E7Lactobatillus caseiPgsA
ISSN
0020-7136
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/ijc.22035
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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