TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling = Rap-1조절에 의한 TIMP-2의 세포 부착 유도 연구

Cited 8 time in scopus
Metadata Downloads
Title
TIMP-2 promotes cell spreading and adhesion via upregulation of Rap1 signaling = Rap-1조절에 의한 TIMP-2의 세포 부착 유도 연구
Author(s)
H Chang; J Lee; Haryoung Poo; M Noda; T Diaz; B Wei; W G Stetler-Stevenson; J Oh
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 345, no. 3, pp. 1201-1206
Publication Year
2006
Abstract
We previously demonstrated that TIMP-2 treatment of human microvascular endothelial cells (hMVECs) activates Rap1 via the pathway of paxillin-Crk-C3G. Here, we show that TIMP-2 overexpression in hMVECs by adenoviral infection enhances Rap1 expression, leading to further increase in Rap1-GTP. TIMP-2 expression, previously reported to inhibit cell migration, also leads to cell spreading accompanied with increased cell adhesion. HMVECs stably expressing Rap1 display a similar phenotype as hMVECs-TIMP-2, whereas the expression of inactive Rap1 mutant, Rap1(38N), leads to elongated appearance with greatly reduced cell adhesion. Furthermore, the phenotype of hMVECs-Rap1(38N) was not reversed by TIMP-2 overexpression. TIMP-2 greatly promotes the association of Rap1 with actin. Therefore, these findings suggest that TIMP-2 mediated alteration in cell morphology requires Rap1, TIMP-2 may recruit Rap1 to sites of actin cytoskeleton remodeling necessary for cell spreading, and enhanced cell adhesion by TIMP-2 expression may hinder cell migration.
Keyword
cell adhesioncell migrationcell spreadingRap1TIMP-2
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2006.05.044
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.