A potential demerit of the pronuclear microinjection technique

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dc.contributor.authorA G Wang-
dc.contributor.authorSun-Uk Kim-
dc.contributor.authorH B Moon-
dc.contributor.authorByung Hwa Hyun-
dc.contributor.authorKi Hoan Nam-
dc.contributor.authorJ G Suh-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorDae Yeul Yu-
dc.contributor.authorDong Seok Lee-
dc.date.accessioned2017-04-19T09:04:54Z-
dc.date.available2017-04-19T09:04:54Z-
dc.date.issued2006-
dc.identifier.issnI000-0052-
dc.identifier.uri10.5352/JLS.2006.16.4.566ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7503-
dc.description.abstractPronuclear microinjection (PMI) is a primary method for producing transgenic mice and offers a powerful tool for investigating gene function in vivo. The method has several reported advantages and disadvantages. Here, we report another potential shortcoming. The survival rate of fertilized one cell-stage embryos was significantly reduced after PMI procedure (65.4% (1202/1838)). In addition, the proportion of embryos developing to full-term was also significantly lower than that of embryos not undergoing PMI (26.5% (319/1202) vs 41.9% (57/136)). Moreover, 3 out of 21 (14.3%) founder control mice which were non-transgene-carrying littermates of transgenic founders showed histopathological changes in their liver, which was comparable to that in of transgenic lineages (4 out of 27 (14.8%)). In conclusion, the mechanical damages in chromosomes occurring during PMI procedure may be a potential factor influencing phenotypes of transgenic mice.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleA potential demerit of the pronuclear microinjection technique-
dc.title.alternativeA potential demerit of the pronuclear microinjection technique-
dc.typeArticle-
dc.citation.titleKorean Journal of Life Sciences-
dc.citation.number4-
dc.citation.endPage570-
dc.citation.startPage566-
dc.citation.volume16-
dc.contributor.affiliatedAuthorSun-Uk Kim-
dc.contributor.affiliatedAuthorByung Hwa Hyun-
dc.contributor.affiliatedAuthorKi Hoan Nam-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.affiliatedAuthorDae Yeul Yu-
dc.contributor.affiliatedAuthorDong Seok Lee-
dc.contributor.alternativeNameWang-
dc.contributor.alternativeName김선욱-
dc.contributor.alternativeName문형배-
dc.contributor.alternativeName현병화-
dc.contributor.alternativeName남기환-
dc.contributor.alternativeName서준교-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName유대열-
dc.contributor.alternativeName이동석-
dc.identifier.bibliographicCitationKorean Journal of Life Sciences, vol. 16, no. 4, pp. 566-570-
dc.identifier.doi10.5352/JLS.2006.16.4.566-
dc.subject.keywordnuclear damage-
dc.subject.keywordpronuclear microinjection-
dc.subject.keywordtransgenic mice-
dc.subject.localnuclear damage-
dc.subject.localpronuclear microinjection-
dc.subject.localtransgenic mice-
dc.subject.localTransgenic mice-
dc.subject.localTransgenic mouse-
dc.subject.localtransgenic mouse-
dc.description.journalClassN-
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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