Principal component analysis and discriminant analysis (PCA-DA) for discriminating profiles of terminal restriction fragment length polymorphism (T-RFLP) in soil bacterial communities

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dc.contributor.authorS Park-
dc.contributor.authorY K Ku-
dc.contributor.authorM J Seo-
dc.contributor.authorDo Young Kim-
dc.contributor.authorJ E Yeon-
dc.contributor.authorK M Lee-
dc.contributor.authorSoon Chun Jeong-
dc.contributor.authorWoon Kee Yoon-
dc.contributor.authorC H Harn-
dc.contributor.authorHwan Mook Kim-
dc.date.accessioned2017-04-19T09:04:54Z-
dc.date.available2017-04-19T09:04:54Z-
dc.date.issued2006-
dc.identifier.issn00380717-
dc.identifier.uri10.1016/j.soilbio.2006.02.019ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7505-
dc.description.abstractTo assess the impact of a transgenic crop on soil environment, we compared soil bacterial communities from the rhizospheres of cucumber green mottle mosaic virus (CGMMV)-resistant transgenic watermelon (Citrullus vulgaris [Twinser] cv. Gongdae) and non-transgenic parental line watermelon at an experimental farm in Miryang, Korea. Soil microbial community structure was studied using terminal restriction fragment length polymorphism (T-RFLP) using HaeIII and HhaI enzymes on products from polymerase chain amplification reactions (PCR) of total DNA from rhizosphere. We used principal component analyses (PCA) to reduce dimensionality of T-RFLP profiles before comparison. On these PCA scores, we conducted discrimination analyses to compare soil microbial communities from the rhizosphere of transgenic and non-transgenic. Discriminant analyses indicate that microbial communities from rhizosphere of transgenic and non-transgenic watermelon did not differ with significance at 95% level. Our study could be used as a model case to assess the environmental risk assessment of transgenic crops on soil microbial organisms.-
dc.publisherElsevier-
dc.titlePrincipal component analysis and discriminant analysis (PCA-DA) for discriminating profiles of terminal restriction fragment length polymorphism (T-RFLP) in soil bacterial communities-
dc.title.alternativePrincipal component analysis and discriminant analysis (PCA?DA) for discriminating profiles of terminal restriction fragment length polymorphism (T-RFLP) in soil bacterial communities-
dc.typeArticle-
dc.citation.titleSoil Biology & Biochemistry-
dc.citation.number8-
dc.citation.endPage2349-
dc.citation.startPage2344-
dc.citation.volume38-
dc.contributor.affiliatedAuthorDo Young Kim-
dc.contributor.affiliatedAuthorSoon Chun Jeong-
dc.contributor.affiliatedAuthorWoon Kee Yoon-
dc.contributor.alternativeName박상규-
dc.contributor.alternativeName구윤경-
dc.contributor.alternativeName서미자-
dc.contributor.alternativeName김도영-
dc.contributor.alternativeName연지은-
dc.contributor.alternativeName이경민-
dc.contributor.alternativeName정순천-
dc.contributor.alternativeName윤원기-
dc.contributor.alternativeName한지학-
dc.contributor.alternativeName김환묵-
dc.identifier.bibliographicCitationSoil Biology & Biochemistry, vol. 38, no. 8, pp. 2344-2349-
dc.identifier.doi10.1016/j.soilbio.2006.02.019-
dc.subject.keywordbacterial community-
dc.subject.keywordcitrullus vulgaris [Twinser] cv. gongdae-
dc.subject.keyworddiscriminant analysis-
dc.subject.keywordPCA-
dc.subject.keywordrhizosphere-
dc.subject.keywordT-RFLP-
dc.subject.keywordtransgenic watermelon-
dc.subject.localbacterial community-
dc.subject.localBacterial community-
dc.subject.localcitrullus vulgaris [Twinser] cv. gongdae-
dc.subject.localdiscriminant analysis-
dc.subject.localDiscriminant analysis (DA)-
dc.subject.localPCA-
dc.subject.localrhizosphere-
dc.subject.localRhizosphere-
dc.subject.localT-RFLP-
dc.subject.localtransgenic watermelon-
dc.subject.localTransgenic watermelon-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Bio-Evaluation Center > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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