Anti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of Inonotus obliquus

Cited 157 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorY O Kim-
dc.contributor.authorH W Park-
dc.contributor.authorJ H Kim-
dc.contributor.authorJ Y Lee-
dc.contributor.authorSeong Hoon Moon-
dc.contributor.authorC S Shin-
dc.date.accessioned2017-04-19T09:04:56Z-
dc.date.available2017-04-19T09:04:56Z-
dc.date.issued2006-
dc.identifier.issn0024-3205-
dc.identifier.uri10.1016/j.lfs.2005.12.047ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7515-
dc.description.abstractThe endo-polysaccharide extracted from mycelia of Inonotus obliquus (Pers.:Fr.) Pil. (Hymenochaetaceae) is a specific activator of B cells and macrophages. However, the in vivo anti-cancer effects and the chemical structure of the endo-polysaccharide are unknown. We purified the endo-polysaccharide, investigated its anti-cancer effects via in vitro and in vivo assays, and performed a structural characterization. The endo-polysaccharide was extracted from I. obliquus mycelia cultivated in a 300-l pilot fermenter, followed by hot water extraction and ethanol precipitation. Purification was achieved by DEAE-cellulose ion-exchange chromatography and gel-permeation chromatography. Chemical analysis revealed that the purified endo-polysaccharide is an α-linked fucoglucomannan with a molecular weight of approximately 1000 kDa. The anti-cancer activities of the endo-polysaccharide against various types of tumor cells were determined. No direct toxicity against either cancer or normal cells was observed. Intraperitoneal administration of the endo-polysaccharide significantly prolonged the survival rate of B16F10-implanted mice, resulting in a 4.07-fold increase in the survival rate at a dose of 30 mg/kg/day. After 60 days of feeding, approximately 67% of the initial number of mice survived with no tumor incidence based on macroscopic examination. These results indicate that the anti-cancer effect of endo-polysaccharide is not directly tumorcidal but rather is immuno-stimulating.-
dc.publisherElsevier-
dc.titleAnti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of Inonotus obliquus-
dc.title.alternativeAnti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of Inonotus obliquus-
dc.typeArticle-
dc.citation.titleLife Sciences-
dc.citation.number1-
dc.citation.endPage80-
dc.citation.startPage72-
dc.citation.volume79-
dc.contributor.affiliatedAuthorSeong Hoon Moon-
dc.contributor.alternativeName김용옥-
dc.contributor.alternativeName박해웅-
dc.contributor.alternativeName김종훈-
dc.contributor.alternativeName이재영-
dc.contributor.alternativeName문성훈-
dc.contributor.alternativeName신철수-
dc.identifier.bibliographicCitationLife Sciences, vol. 79, no. 1, pp. 72-80-
dc.identifier.doi10.1016/j.lfs.2005.12.047-
dc.subject.keywordAnti-cancer activity-
dc.subject.keywordChemical structure-
dc.subject.keywordEndo-polysaccharide-
dc.subject.keywordImmuno-stimulating-
dc.subject.keywordInonotus obliquus-
dc.subject.localAnti-cancer activity-
dc.subject.localAnticancer activity-
dc.subject.localanticancer activity-
dc.subject.localChemical structure-
dc.subject.localChemical Structures-
dc.subject.localchemical structure-
dc.subject.localEndo-polysaccharide-
dc.subject.localImmuno-stimulating-
dc.subject.localInonotus obliquus-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.