Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy

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Title
Observation of self-assembled fluorescent beads by scanning near-field optical microscopy and atomic force microscopy
Author(s)
Y J Oh; W Jo; Min-Gon Kim; Hyun Kyu Park; Bong Hyun Chung
Bibliographic Citation
Ultramicroscopy, vol. 106, no. 8, pp. 775-778
Publication Year
2006
Abstract
Optical response and topography of fluorescent latex beads both on flat self-assembled monolayer and on a micron-patterned surface with poly(dimethylsiloxane) are studied. Scanning near-field optical microscopy and atomic force microscopy were utilized together for detecting fluorescence and imaging topography of the patterned latex beads, respectively. As a result, the micro-patterned latex beads where a specific chemical binding occurred show a strong signal, whereas no signals are observed in the case of nonspecific binding. With fluorescein isothiocyanate (FITC), it is convenient to measure fluorescence signal from the patterned beads allowing us to monitor the small balls of fluorescent latex.
Keyword
BeadsFluorescence detectionSelf-assembled monolayersSNOM
ISSN
0304-3991
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ultramic.2005.12.014
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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