Production of transgenic birdsfoot trefoil plants by introduction of 'SWPA2 promoter + AtNDPK2 gene' = 'SWPA2 프로모터 + AtNDPK2 유전자' 도입에 의한 버즈풋 트레포일 형질전환체 생산

Abstract

To develop transgenic birdsfoot trefoil (Lotus corniculatus L.) plants tolerant to environmental stress, Arabidopsis NDPK gene (AtNDPK) was introduced into birdsfoot trefoil plants using Agrobacterium-mediated transformation and expressed powerfully under the control of the SWPA2 promoter. The expression vector, pCAMBIA2300 was used for introduction of AtNDPK gene into birdsfoot trefoil plants. The transformed calli were selected on kanamycin containing medium and then regenerated. The transformed birdsfoot trefoil plants were cultivated for 4 months on BOi2Y medium.