The epigenetic silencing of LIMS2 in gastric cancer and its inhibitory effect on cell migration

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dc.contributor.authorSeung Kyoon Kim-
dc.contributor.authorHae-Ran Jang-
dc.contributor.authorJeong Hwan Kim-
dc.contributor.authorS M Noh-
dc.contributor.authorK S Song-
dc.contributor.authorMirang Kim-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorYoung Il Yeom-
dc.contributor.authorNam-Soon Kim-
dc.contributor.authorHyang Sook Yoo-
dc.contributor.authorYong Sung Kim-
dc.date.accessioned2017-04-19T09:05:05Z-
dc.date.available2017-04-19T09:05:05Z-
dc.date.issued2006-
dc.identifier.issn0006-291X-
dc.identifier.uri10.1016/j.bbrc.2006.08.128ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7572-
dc.description.abstractRecent finding has shown that LIMS2 (also known as PINCH2) functions as a natural regulator of the LIMS1-ILK-parvin complex formation and is associated with cell spreading and migration via integrins at focal adhesions. Here, we report for the first time the epigenetic silencing of LIMS2 in gastric tumors. Downregulation of LIMS2 was detected in 91% (10 of 11) of gastric cancer cell lines by real-time quantitative RT-PCR and 80% (8 of 10) of the LIMS2-downregulated cell lines were associated with CpG island hypermethylation at a 5′-upstream region of LIMS2. Furthermore, LIMS2 was restored in its non-expressing cell lines after treatment with 5-Aza-dC and/or trichostatin A. Loss of expression of LIMS2 was also detected in 53% (51 of 96) of primary gastric tumors. This decrease in expression level significantly correlated with an increase of the CpG island hypermethylation. In addition, the methylation status in any normal-appearing gastric tissues was gradually increased in an age-dependent manner, suggesting that the positive methylation in normal-appearing gastric mucosa can be due to 'field cancerization effect' as an early event in gastric carcinogenesis. Moreover, the transient transfection of LIMS2-siRNA significantly stimulated cell migration in gastric cancer cells but had no effects on cell growth. These results suggest that the frequent inactivation of LIMS2 by epigenetic alteration in gastric cancer may be important in tumor progression events, such as invasion and metastasis. Thus, LIMS2 may be useful as a molecular biomarker and a therapeutic target by increasing its expression and activity in gastric cancer.-
dc.publisherElsevier-
dc.titleThe epigenetic silencing of LIMS2 in gastric cancer and its inhibitory effect on cell migration-
dc.title.alternativeThe epigenetic silencing of LIMS2 in gastric cancer and its inhibitory effect on cell migration-
dc.typeArticle-
dc.citation.titleBiochemical and Biophysical Research Communications-
dc.citation.number3-
dc.citation.endPage1040-
dc.citation.startPage1032-
dc.citation.volume349-
dc.contributor.affiliatedAuthorSeung Kyoon Kim-
dc.contributor.affiliatedAuthorHae-Ran Jang-
dc.contributor.affiliatedAuthorJeong Hwan Kim-
dc.contributor.affiliatedAuthorMirang Kim-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.affiliatedAuthorNam-Soon Kim-
dc.contributor.affiliatedAuthorHyang Sook Yoo-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName김승균-
dc.contributor.alternativeName장해란-
dc.contributor.alternativeName김정환-
dc.contributor.alternativeName노승모-
dc.contributor.alternativeName송규상-
dc.contributor.alternativeName김미랑-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName염영일-
dc.contributor.alternativeName김남순-
dc.contributor.alternativeName유향숙-
dc.contributor.alternativeName김용성-
dc.identifier.bibliographicCitationBiochemical and Biophysical Research Communications, vol. 349, no. 3, pp. 1032-1040-
dc.identifier.doi10.1016/j.bbrc.2006.08.128-
dc.subject.keywordgastric cancer-
dc.subject.keywordLIMS2-
dc.subject.keywordmethylation-
dc.subject.keywordmigration-
dc.subject.localgastric cancer-
dc.subject.localGastric cancer (GC)-
dc.subject.localGastric cancer-
dc.subject.localLIMS2-
dc.subject.localmethylation-
dc.subject.localMethylation-
dc.subject.localMigration-
dc.subject.localmigration-
dc.description.journalClassY-
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
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