On-chip Escherichia coli culture, purification, and detection of expressed proteins

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dc.contributor.authorMoonil Kim-
dc.contributor.authorSo Young Lee-
dc.contributor.authorHyun-Ju Choi-
dc.contributor.authorYong Beom Shin-
dc.contributor.authorSun Ok Jung-
dc.contributor.authorMin-Gon Kim-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T09:05:08Z-
dc.date.available2017-04-19T09:05:08Z-
dc.date.issued2006-
dc.identifier.issn0175-7571-
dc.identifier.uri10.1007/s00249-006-0072-8ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7580-
dc.description.abstractIn a recent study, we reported the results of a rapid high-throughput expression analysis of the affinity-tagged proteins present in total cell lysates, using a surface plasmon resonance (SPR) imaging protein chip system. In this paper, we describe a novel method, which is able to sequentially carry out a recombinant Escherichia coli culture, as well as the detection and purification of the expressed proteins on a single microwell chip, fabricated on a two-dimensional thin gold film. Following the induction of the protein on the microwell chip, the E. coli cells were lysed on the chip via the addition of lysozymes, and the expressed glutathione S-transferase-fused green fluorescent protein (GST-GFP) was then purified on the chip via affinity interaction with the glutathionylated gold surface of the chip. Finally, the expressed protein was directly detected using the surface plasmon resonance (SPR) imaging system. This system saves a substantial amount of time, experimental resources, and labor, by allowing for the complicated and labor-intensive procedures inherent to the production of recombinant proteins to be conducted on a single microwell chip, simply and economically.-
dc.publisherSpringer-
dc.titleOn-chip Escherichia coli culture, purification, and detection of expressed proteins-
dc.title.alternativeOn-chip Escherichia coli culture, purification, and detection of expressed proteins-
dc.typeArticle-
dc.citation.titleEuropean Biophysics Journal with Biophysics Letters-
dc.citation.number8-
dc.citation.endPage662-
dc.citation.startPage655-
dc.citation.volume35-
dc.contributor.affiliatedAuthorMoonil Kim-
dc.contributor.affiliatedAuthorYong Beom Shin-
dc.contributor.affiliatedAuthorSun Ok Jung-
dc.contributor.affiliatedAuthorMin-Gon Kim-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName김문일-
dc.contributor.alternativeName이소영-
dc.contributor.alternativeName최현주-
dc.contributor.alternativeName신용범-
dc.contributor.alternativeName정선옥-
dc.contributor.alternativeName김민곤-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationEuropean Biophysics Journal with Biophysics Letters, vol. 35, no. 8, pp. 655-662-
dc.identifier.doi10.1007/s00249-006-0072-8-
dc.subject.keywordGlutathione S-transferase-fused green fluorescent protein (GST-GFP)-
dc.subject.keywordMicrowell chip-
dc.subject.keywordOn-chip purification-
dc.subject.keywordSurface plasmon resonance (SPR) imaging system-
dc.subject.localGlutathione S-transferase-fused green fluorescent protein (GST-GFP)-
dc.subject.localMicrowell chip-
dc.subject.localmicrowell chip-
dc.subject.localOn-chip purification-
dc.subject.localSurface plasmon resonance (SPR) imaging system-
dc.description.journalClassY-
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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