Simple method for RNA preparation from cyanobacteria

Cited 13 time in scopus
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Title
Simple method for RNA preparation from cyanobacteria
Author(s)
Byung-Hyuk Kim; Hee-Mock Oh; Young Ki Lee; Gang Guk Choi; Chi-Yong Ahn; Byung Dae Yoon; Hee-Sik Kim
Bibliographic Citation
Journal of Phycology, vol. 42, no. 5, pp. 1137-1141
Publication Year
2006
Abstract
A simple and rapid method is presented for the preparation of RNA from various cyanobacteria. Unlike other methods that require a lysis solution, lysozymes, or proteinase K, the proposed method, called the bead-phenol- chloroform (BPC) method, uses silica/zirconia beads, phenol, and chloroform to break the cells and extract RNA more efficiently. Experiments confirm that the BPC method can successfully isolate total RNA from various cyanobacterial strains without DNA contamination, and the extracted RNA samples have a relatively high purity, concentration, and yield. Furthermore, the BPC method is more rapid, simple, and economical when compared with previously reported methods.
Keyword
bead-phenol-chloroform methodcyanobacteriaRNA extractionxanthogenate-SDS-phenol method
ISSN
0022-3646
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1529-8817.2006.00263.x
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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