Glaciecola nitratireducens sp. nov., isolated from seawater

Abstract

A marine bacterial strain, FR1064T, was isolated from a surface seawater sample collected off Jeju Island, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the Gammaproteobacteria and was related to the genus Glaciecola with 97.6% sequence similarity to Glaciecola pallidula, its nearest phylogenetic neighbour. DNA-DNA relatedness between strain FR1064T and G. paffidula ACAM 615T was 55%. Cells of the novel isolate were Gram-negative, aerobic, rod-shaped, motile and halophilic, with an optimum sea salts concentration of 4-7%. The major fatty acids were straight-chain saturated (C16:0), summed feature 3 and monounsaturated fatty acid C18:1. The DNA G + C content was 44 mol%. Several phenotypic characteristics differentiated the novel isolate from all previously described members of the genus Glaciecola. The polyphasic data obtained in this study clearly demonstrate that strain FR1064T represents a novel species of the genus Glaciecola. The name Glaciecola nitratireducens sp. nov. is therefore proposed, with strain FR1064T (=KCTC 12276T=JCM 12485T) as the type strain.