Gene disruption using In vivo and In vitro methylation in Streptomyces griseus

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Title
Gene disruption using In vivo and In vitro methylation in Streptomyces griseus
Author(s)
J S Maeng; Kyung Sook Bae; Jangryul Kwak
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 16, no. 9, pp. 1472-1476
Publication Year
2006
Abstract
Previous study demonstrated that the restriction barrier of Streptomyces griseus is almost completely bypassed by the Streptomyces-E. coli shuttle vectors passed through the E. coli GM161 strain and methylated with AluII and HpaII methyltransferases. The same DNA methylation of the genomic DNA fragments cloned the nonreplicative vectors generated integrative transformation and gene disruption of their chromosomal counterparts at high efficiencies in S. griseus. This result indicated that the efficiency of gene disruption depends on the efficient transfer of the incoming DNA into bacterial hosts.
Keyword
gene disruptionhomologous recombinationmethylationstreptomyces griseustransformation
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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