Inhibitory effect of peroxiredoxin II (Prx II) on Ras-ERK-NFκB pathway in mouse embryonic fibroblast (MEF) senescence

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Title
Inhibitory effect of peroxiredoxin II (Prx II) on Ras-ERK-NFκB pathway in mouse embryonic fibroblast (MEF) senescence
Author(s)
Y H Han; Jung Hun Kwon; Dae Yeul Yu; E Y Moon
Bibliographic Citation
Free Radical Research, vol. 40, no. 11, pp. 1182-1189
Publication Year
2006
Abstract
Intracellular reactive oxygen species (ROS) were attenuated by the expression of peroxiredoxin II (Prx II). Cellular senescence as judged by senescence-associated (SA)-β-galactosidase (Gal) positive cell formation was increased in Prx II-deficient mouse embryonic fibroblast (MEF). Ras expression was increased following passages. The level of Ras expression was higher in Prx II-/- MEF than wild type MEF. ERK activity was also augmented by the deletion of Prx II. SA-β-Gal-positive cell formation was reduced by PD98059, ERK inhibitor. Activated nuclear transcription factor, nuclear factor-kappaB (NFκB) by the deletion of Prx II was inhibited by the treatment with PD98059. In contrast, no changes in SA-β-Gal-positive cell formation were detected by NFκB inhibitor, N-alpha-tosyl-l-phenylalanyl chloromethyl ketone (TPCK). Collectively, results suggest that Prx II deletion activate Ras-ERK-NFκB pathways and cellular senescence in Prx II-/- MEF cells was mediated by ERK activation but not by NFκB activation.
Keyword
ERKMEFNFκBPeroxiredoxin IIRasROS
ISSN
1071-5762
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1080/10715760600868552
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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