Structure, expression, and mapping of two nodule-specific genes identified by mining public soybean EST databases = 콩 EST 정보 분석에 의해 동정된 두 뿌리혹 특이 유전자의 구조, 발현 및 유전자 지도화

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dc.contributor.authorSoon Chun Jeong-
dc.contributor.authorKiwoung Yang-
dc.contributor.authorJi-Young Park-
dc.contributor.authorK S Han-
dc.contributor.authorSeok Yu-
dc.contributor.authorT Y Hwang-
dc.contributor.authorCheol-Goo Hur-
dc.contributor.authorSeung Ho Kim-
dc.contributor.authorP B Park-
dc.contributor.authorHwan Mook Kim-
dc.contributor.authorY I Park-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T09:05:21Z-
dc.date.available2017-04-19T09:05:21Z-
dc.date.issued2006-
dc.identifier.issn03781119-
dc.identifier.uri10.1016/j.gene.2006.07.015ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/7611-
dc.description.abstractNumerous nodule-specific genes, which are involved in the root nodule development and function, have been known and are still being discovered. Here, we reported the structure, expression, and genetic map location of two novel nodule-specific genes. First, two EST groups, one obtained from a nodule library and the other from all aboveground tissue libraries, were clustered with regard to in silico expression profiles. We compiled a pool of 103 putative nodule-specific sequence clusters. Then, two representative ESTs were selected for further experimental analyses. According to the full-length cDNA sequences, one was an EST of a novel nodule-specific polygalacturonase gene, GmPGN, and the other was an EST of a new short nodule-specific gene, GmEKN. The results of expression analyses of the GmPGN cDNAs indicated that GmPGN expression was not detectable in any of the soybean tissues except in the nodule tissue and may be regulated via alternative splicing. GmEKN expression was the most strongly detected in the nodule. The predicted GmEKN protein is both glutamic acid- and lysine-rich, and is also highly hydrophilic. Genetic mapping located GmPGN near the known quantitative trait locus conferring resistance to soybean cyst nematode on soybean molecular linkage group (MLG) B1, and GmEKN on MLG A2. These results provide useful information for the use of these genes in research on the orchestration of numerous genes in nodule development and function.-
dc.publisherElsevier-
dc.titleStructure, expression, and mapping of two nodule-specific genes identified by mining public soybean EST databases = 콩 EST 정보 분석에 의해 동정된 두 뿌리혹 특이 유전자의 구조, 발현 및 유전자 지도화-
dc.title.alternativeStructure, expression, and mapping of two nodule-specific genes identified by mining public soybean EST databases-
dc.typeArticle-
dc.citation.titleGene-
dc.citation.number1-
dc.citation.endPage80-
dc.citation.startPage71-
dc.citation.volume383-
dc.contributor.affiliatedAuthorSoon Chun Jeong-
dc.contributor.alternativeName정순천-
dc.contributor.alternativeName양기웅-
dc.contributor.alternativeName박지영-
dc.contributor.alternativeName한기수-
dc.contributor.alternativeName유석-
dc.contributor.alternativeName황태영-
dc.contributor.alternativeName허철구-
dc.contributor.alternativeName김승호-
dc.contributor.alternativeName박훤범-
dc.contributor.alternativeName김환묵-
dc.contributor.alternativeName박연일-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationGene, vol. 383, no. 1, pp. 71-80-
dc.identifier.doi10.1016/j.gene.2006.07.015-
dc.subject.keywordEST-
dc.subject.keywordglycine max-
dc.subject.keywordGmEKN-
dc.subject.keywordGmPGN-
dc.subject.keywordnodulins-
dc.subject.localEST-
dc.subject.localglycine max-
dc.subject.localGlycine max-
dc.subject.localGmEKN-
dc.subject.localGmPGN-
dc.subject.localnodulins-
dc.description.journalClassY-
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Ochang Branch Institute > 1. Journal Articles
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