Investigation of the doughnut effect in Cy3-Labeled protein microarrays using scanning near-field optical microscope

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Title
Investigation of the doughnut effect in Cy3-Labeled protein microarrays using scanning near-field optical microscope
Author(s)
A Gokarna; Y H Kim; Y H Cho; M S Lee; I C Kang; Hyun Kyu Park; Min-Gon Kim; Bong Hyun Chung
Bibliographic Citation
Optical Review, vol. 13, no. 4, pp. 288-291
Publication Year
2006
Abstract
We demonstrate the fluorescence mapping of protein microarrays by the technique of scanning near-field optical microscopy (SNOM) and confocal microscopy. Micron sized spots (300 μm) of human Immunoglobulin G (hIgG) protein with and without a Cy3 dye labeling have been fabricated on glass substrates by an immobilization method which makes use of calixcrown derivatives termed Prolinker. We have also tried to probe into the well-known "doughnut effect" observed in fluorescence images of proteins using the SNOM technique. The topographic and fluorescence SNOM images revealed that the number of proteins at the boundary of the spot were more than at the center in the case of the microarray spot which showed brighter luminescence at the edge than at the center in the confocal image.
Keyword
confocal microscopyfluorescenceprotein chipprotein microarrayscanning near field optical microscopy
ISSN
1340-6000
Publisher
Optical Soc Japan
DOI
http://dx.doi.org/10.1007/s10043-006-0288-y
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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