Polyphasic assignment of a highly proteolytic bacterium isolated from a spider to Serratia proteamaculans

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Title
Polyphasic assignment of a highly proteolytic bacterium isolated from a spider to Serratia proteamaculans
Author(s)
Jangryul Kwak; D H Lee; Y D Park; S B Kim; J S Maeng; Hyun Woo OhHo Yong Park; Kyung Sook Bae
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 16, no. 10, pp. 1537-1543
Publication Year
2006
Abstract
A bacterial strain named HY-3 that produces a highly active extracellular protease was isolated from the digestive tract of a spider, Nephila clavata. The bacterium was a Gram-negative, oxidase-negative, catalase-positive, nonhalophilic, nitrate-reducing, facultative anaerobe. Transmission and scanning electron microscopies demonstrated that the isolate was non-spore-forming, straight, rod-shaped, and motile by peritrichous flagella. The G+C content of the DNA was 57.0 mol%. The isoprenoid quinone type was ubiquinone with 8 isoprene units (Q-8). The morphological and biochemical characteristics including the predominant fatty acid and phospholipids profiles placed the isolate HY-3 in the family Enterobacteriaceae. Further biochemical characterization and phylogenetic studies including determination of an almost complete 16S ribosomal DNA sequence suggested that the bacterium was closely related to the genus Serratia. DNA-DNA hybridization analysis revealed that this extracellular protease-producing strain belongs to Serratia proteamaculans, which is also known for its association with insects.
Keyword
phylogenyproteaseserratia proteomaculansspider
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Facility Management Center > 1. Journal Articles
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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