Characterization and selection of lactic acid bacteria producing β-galactosidase = β-galactosidase 생산 유산균 선별 및 특성 조사

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Title
Characterization and selection of lactic acid bacteria producing β-galactosidase = β-galactosidase 생산 유산균 선별 및 특성 조사
Author(s)
Young Ki Lee; S Choi; Y I Park; Chan Sun Park; Byung Dae Yoon; Y S Hwang; Hee-Sik Kim
Bibliographic Citation
Korean Journal of Microbiology, vol. 42, no. 3, pp. 216-222
Publication Year
2006
Abstract
This study was carried out to select the lactic acid bacteria producing β-galactosidase (lactase) and investigate the properties of the β-galactosidase. About 100 strains of lactic acid bacteria showing blue colony on the MRS agar medium containing X-gal were isolated from several kinds of Kimchi. Among them, 2 strains were selected as potential β-galactosidase producers. The selected strains, ET-1 and LA-12, were identified as Lactobacillus fermentum and L. acidophilus, respectively by the analysis of 16S rDNA sequences. They showed relatively high β-galactosidase activity and cellular viability. Their β-galactosidase showed the highest activity at 55oC. And the optimum pHs of the enzymes produced by ET-1 and LA-12 were pH 5.5 and pH 7.0, respectively. They were also highly resistant to artificial gastric juice and bile. Two selected strains showed little change of viable cell number for 3 hr incubation in artificial gastric juice, and maintained the viable cell number at 108 CFU/ml for 24 hr in 0.3% oxgall after incubation for 2 hours in artificial gastric juice. Based on these results, ET-1 and LA-12 are expected to be applied in dairy industry.
Keyword
β-galactosidaselactobacillus내담즙성내산성유산균
ISSN
0440-2413
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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